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05-1527 Anti-eIF4A3 Antibody, clone 3F1

Use Anti-eIF4A3 Antibody, clone 3F1 (mouse monoclonal antibody) validated in WB, IP, ICC to detect eIF4A3 also known as ATP-dependent RNA helicase DDX48, Eukaryotic translation initiation factor 4A isoform 3.

Anti-eIF4A3 Antibody, clone 3F1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: DDX48, EIF4AIII, NMP265, NUK-34, eIF4AIII, hNMP265 UniProt Number: P38919
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05-1527
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IP, ICCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1527
DescriptionAnti-eIF4A3 Antibody, clone 3F1
Alternate Names
  • ATP-dependent RNA helicase DDX48
  • ATP-dependent RNA helicase eIF4A-3
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 48
  • DEAD box protein 48
  • Eukaryotic initiation factor 4A-like NUK-34
  • Eukaryotic translation initiation factor 4A isoform 3
  • Nuclear matrix protein 265
  • eukaryotic translation initiation factor 4A, isoform 3
  • hNMP 265
Background InformationEukaryotic translation initiation factor 4A, isoform 3, also known as eIF4A3, is a member of the DEAD box protein family. DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). This family of proteins are putative RNA helicases and are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. eIF4A3 is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-eIF4A3 Antibody, clone 3F1 (mouse monoclonal antibody) validated in WB, IP, ICC to detect eIF4A3 also known as ATP-dependent RNA helicase DDX48, Eukaryotic translation initiation factor 4A isoform 3.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesImmunoprecipitation: Reported by independent laboratory using representative lot.
Immunocytochemistry: Reported by independent laboratory using representative lot.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human eIF4A3.
EpitopeUnknown
Clone3 F 1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes eIF4A3.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with mouse based on sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq].
Gene Symbol
  • DDX48
  • EIF4AIII
  • NMP265
  • NUK-34
  • eIF4AIII
  • hNMP265
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUCTION: Binds to spliced mRNAs and is involved in nonsense-mediated decay of mRNAs containing premature stop codons.

SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, EIF4A3, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Part of the exon junction complex (EJC), a multisubunit complex which assembles near mRNA exon-exon junctions and which contains proteins involved in mRNA splicing, export, localization and nonsense-mediated decay. May interact with NOM1.

SUBCELLULAR LOCATION: Nucleus.

TISSUE SPECIFICITY: Ubiquitously expressed.

SEQUENCE SIMILARITIES: Belongs to the DEAD box helicase family. eIF4A subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain. ...
Molecular Weight 47 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected eIF4A3 on 10 µg in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-1527 04053252736971

Documentation

Anti-eIF4A3 Antibody, clone 3F1 SDS

Title

Safety Data Sheet (SDS) 

Anti-eIF4A3 Antibody, clone 3F1 Certificates of Analysis

TitleLot Number
Anti-eIF4A3, clone 3F1 - 2225184 2225184
Anti-eIF4A3, clone 3F1 - 2318040 2318040
Anti-eIF4A3, clone 3F1 - 3236371 3236371
Anti-eIF4A3, clone 3F1 - 3507308 3507308
Anti-eIF4A3, clone 3F1 - 3784535 3784535
Anti-eIF4A3, clone 3F1 - NG1804088 NG1804088
Anti-eIF4A3, clone 3F1 - NG1881834 NG1881834
Anti-eIF4A3, clone 3F1 - NRG1672066 NRG1672066
Anti-eIF4A3, clone 3F1 -2541559 2541559
Anti-eIF4A3, clone 3F1 -2570804 2570804
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