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05-1517 Anti-hnRNP Q Antibody, clone 18E4

Use Anti-hnRNP Q Antibody, clone 18E4 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect hnRNP Q also known as heterogeneous nuclear ribonucleoprotein Q-like.

Anti-hnRNP Q Antibody, clone 18E4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: hnRNP-Q, hnRNP Q, SYNCRIP, HNRPQ, NSAP1 UniProt Number: O60506
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05-1517
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1517
DescriptionAnti-hnRNP Q Antibody, clone 18E4
Alternate Names
  • heterogeneous nuclear ribonucleoprotein Q-like
  • Synaptotagmin-binding, cytoplasmic RNA-interacting protein
  • Glycine- and tyrosine-rich RNA-binding protein
  • GRY-RBP
  • NS1-associated protein 1
Background InformationHeterogeneous nuclear ribonucleoprotein Q commoly refered to as hnRNP Q can be found as three different isoforms derived from alternative splicing of a single gene. This RNA binding protein is implicated in control of mRNA precursor splicing for the survival of motor neurons (SMN) protein, a gene. Loss-of-function mutations of SMN are linked to spinal muscular atrophy (SMA) a common neurodegenerative disease. It has been shown that hnRNP Q proteins interact with SMN, and this is interacton is required for efficient pre-mRNA splicing in vitro. Current data suggests that hnRNP Q is a splicing modulator of SMN.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-hnRNP Q Antibody, clone 18E4 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect hnRNP Q also known as heterogeneous nuclear ribonucleoprotein Q-like.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunocytochemistry
Cited by independent researcher using a representative lot.
Immunoprecipitation
Cited by independent researcher using a representative lot.
Biological Information
ImmunogenRecombinant protein corresponding to human hnRNP Q.
EpitopeUnknown
Clone18E4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes hnRNP Q.
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • hnRNP-Q
  • hnRNP Q
  • SYNCRIP
  • HNRPQ
  • NSAP1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 3 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins.

SUBUNIT STRUCTURE: Isoform 1 is a component of the APOB mRNA editosome complex and interacts with APOBEC1 and A1CF (APOBEC1 complementation factor). Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, CSDE1/UNR, HNRPD and SYNCRIP. Isoform 3 interacts with HNRPR. Interacts with POLR2A hyperphosphorylated C-terminal domain. Interacts with minute virus of mice (MVM) NS1 protein. Isoform 1, isoform 2 and isoform 3 interact with SMN. Isoform 3 interacts through its C-terminal domain with SYT7, SYT8 and SYT9 By similarity. The non-phosphorylated and phosphorylated forms are colocalized with PAIP1 in polysomes By similarity. Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1 By similarity. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.

SUBCELLULAR LOCATION: Cytoplasm. Microsome By similarity. Endoplasmic reticulum By similarity. Nucleus By similarity. Note: The tyrosine phosphorylated form bound to RNA is found in microsomes By similarity. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Isoform 1: Nucleus › nucleoplasm By similarity. Note: Expressed predominantly in the nucleoplasm By similarity.

Isoform 2: Nucleus › nucleoplasm By similarity. Note: Expressed predominantly in the nucleoplasm By similarity.

Isoform 3: Nucleus › nucleoplasm By similarity. Note: Expressed predominantly in the nucleoplasm By similarity.

TISSUE SPECIFICITY: Ubiquitously expressed. Detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.

Domain The domain containing eight Arg-Gly-Gly repeats may be involved in RNA-binding and protein-protein interactions.

PTM: Phosphorylated on tyrosine. The membrane-bound form found in microsomes is phosphorylated in vitro by insulin receptor tyrosine kinase (INSR). Phosphorylation is inhibited upon binding to RNA, whereas the cytoplasmic form is poorly phosphorylated By similarity. Phosphorylated upon DNA damage, probably by ATM or ATR.

SEQUENCE SIMILARITIES: Contains 3 RRM (RNA recognition motif) domains.

SEQUENCE CAUTION: The sequence AAH15575.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence starting in position 413.
Molecular WeightThe calculated molecular weight is 70 kDa. Clone 18E4 can recognizes four bands on the Western Blot of total HeLa cell lysate corresponding to molecular masses of ~80, ~70, ~60 and ~55 kDa. hnRNP Q has three isoforms of ~70– 55 kDa. The ~80 kDa reactive band was identified as hnRNP R, which migrates at ~80 kDa on SDS–PAGE. (Mourelatos., Z., et al., EMBO J., 20, 5443-5452 (2001).)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected hnRNP Q on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-1517 04053252333293

Documentation

Anti-hnRNP Q Antibody, clone 18E4 SDS

Title

Safety Data Sheet (SDS) 

Anti-hnRNP Q Antibody, clone 18E4 Certificates of Analysis

TitleLot Number
Anti-hnRNP Q, clone 18E4 - 2387782 2387782
Anti-hnRNP Q, clone 18E4 - 2450697 2450697
Anti-hnRNP Q, clone 18E4 - 2293525 2293525
Anti-hnRNP Q, clone 18E4 - 2526828 2526828
Anti-hnRNP Q, clone 18E4 - 3227311 3227311
Anti-hnRNP Q, clone 18E4 - 3485009 3485009
Anti-hnRNP Q, clone 18E4 - 3979348 3979348
Anti-hnRNP Q, clone 18E4 - NRG1690167 NRG1690167
Anti-hnRNP Q, clone 18E4 Monoclonal Antibody 2949952
Anti-hnRNP Q, clone 18E4 Monoclonal Antibody 3015365