Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABN527 Anti-iNOS/NOS II, clone 13F5.1 Antibody

This Anti-iNOS/NOS II antibody is validated for use in WB, IH for the detection of iNOS/NOS II.

Anti-iNOS/NOS II, clone 13F5.1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: NOS2, NOS2A UniProt Number: P35228
View Products on Sigmaaldrich.com
MABN527
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABN527
DescriptionAnti-iNOS/NOS II, clone 13F5.1 Antibody
Alternate Names
  • Nitric oxide synthase, inducible
  • Hepatocyte NOS
  • HEP-NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • NOS type II
  • Peptidyl-cysteine S-nitrosylase NOS2
Background InformationiNOS/NOS II, also known as Nitric oxide synthase inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase , and encoded by the gene name NOS2 and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2). iNOS/NOS II is expressed in the liver, retina, bone cells and airway epithelial cells of the lung.
References
Product Information
FormatCulture Supernatant
PresentationMouse monoclonal IgG1λ in buffer containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-iNOS/NOS II antibody is validated for use in WB, IH for the detection of iNOS/NOS II.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected iNOS/NOS II in human cerebral cortex, human cerebellum, and human colon tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human iNOS/NOS II.
Clone13F5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1λ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOS2
  • NOS2A
Purification MethodUnpurified
UniProt Number
Molecular Weight~130 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human small intestine tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected iNOS/NOS II in 10 µg of human small Intestine tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABN527 04055977121629

Documentation

Anti-iNOS/NOS II, clone 13F5.1 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-iNOS/NOS II, clone 13F5.1 Antibody Certificates of Analysis

TitleLot Number
Anti-iNOS/NOS II, clone 13F5.1 - 3190915 3190915
Anti-iNOS/NOS II, clone 13F5.1 - 3660367 3660367
Anti-iNOS/NOS II, clone 13F5.1 -2769034 2769034
Anti-iNOS/NOS II, clone 13F5.1 -2809948 2809948
Anti-iNOS/NOS II, clone 13F5.1 -Q2488078 Q2488078
Anti-iNOS/NOS II, clone 13F5.1 Monoclonal Antibody 2991410

Brochure

Title
Pathways and Biomarkers of Oxidative Stress

Technical Info

Title
White Paper- Modern Methods in Oxidative Stress Research