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MABS2313-25UG Anti-iRhom1 Antibody, clone RHF1A 20A8

Anti-iRhom1, clone RHF1A 20A8, Cat. No. MABS2313, is a rat monoclonal antibody that detects iRhom1 and is tested for use in Western Blotting.

NewMABS2313-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABS2313-25UG
DescriptionAnti-iRhom1 Antibody, clone RHF1A 20A8
Alternate Names
  • Inactive rhomboid protein 1
  • Epidermal growth factor receptor-related protein
  • Rhomboid 5 homolog 1
  • Rhomboid family member 1
  • p100hRho
Background InformationInactive rhomboid protein 1 (UniProt: Q96CC6; also known as iRhom1, Epidermal growth factor receptor-related protein, Rhomboid 5 homolog 1, Rhomboid family member 1, p100hRho, RHBDF1) is encoded by the RHBDF1 (also known as C16orf8, DIST1, IRHOM1) gene (Gene ID: 64285) in human. iRhom1 is a multi-pass, homodimeric, membrane protein that is predominantly localized in the endoplasmic reticulum (ER). It is ubiquitously expressed, but higher expression is observed in brain. It regulates the maturation and activity of ADAM17, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF . It also controls the transport of ADAM17 from ER to the plasma membrane and removal of its inhibitory propeptide, and stability and degradation. It is reported to play a role in cell survival, proliferation, migration, and inflammation. However, it does not exhibit any protease activity on its own due to lack of amino acids that are required for proteolytic activity. iRhom1-deficient mice are reported to lack mature ADAM17 in the brain. (Ref.: Tüshaus, J., et al. (2021). FASEB J. 35(11); e21962; Weskamp, G., et al. (2020). J. Biol. Chem. 295(23); 4350-4358).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-iRhom1, clone RHF1A 20A8, Cat. No. MABS2313, is a rat monoclonal antibody that detects iRhom1 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
  • Western Blot Using KO Lysates
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected iRhom1 in Western Blotting applications (Weskamp, G., et al. (2020). J Biol Chem. 295(13):4350-4358).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected iRhom1 in Mouse embryonic fibroblasts (MEF) lysates, but not in lysates with iRhom1 knockout (Courtesy of Katrin Moschke, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE), Munich, Germany).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human Inactive rhomboid protein 1 (iRhom1).
EpitopeN-terminal
CloneRHF1A 20A8
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone RHF1A 20A8 is a rat monoclonal antibody that detects Inactive rhomboid protein 1 (iRhom1). It targets an epitope within 19 amino acids from the N-terminal region.
IsotypeIgG2a
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RHBDF1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~97 kDa observed; 97.4 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in 721.221 cell lysate.

Western Blotting Analysis: A 1:125 dilution of this antibody detected iRhom1 in 721.221 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS2313-25UG 04065270938077