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MAB131873 Anti-mCherry Antibody, clone 1C51

Anti-mCherry Antibody, clone IC51, Cat. No. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting.

Anti-mCherry Antibody, clone 1C51 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Gene Symbol: mCherry UniProt Number: X5DSL3
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MAB131873
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB131873
DescriptionAnti-mCherry Antibody, clone 1C51
Background InformationmCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. It is monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, clone1C51 is an affinity purified antibody that recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et al. (2004). Nat. Biotechnol. 22(12):1567-72).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing PBS with 0.03% sodium azide with 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-mCherry Antibody, clone IC51, Cat. No. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected mCherry in Western Blotting applications (Krajcovic, M., et. al. (2013). Mol Biol Cell. 24(23):3736-45).

Western Blotting Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vs HEK293 untransfected cells (Courtesy of Dr Gerry Shaw).

Immunocytochemistry Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry (Courtesy of Dr Gerry Shaw).
Biological Information
ImmunogenHis-tagged full length recombinant mCherry.
Clone1C51
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1C51 detects mCherry protein in multiple species.
IsotypeIgG2aκ
Species Reactivity
  • All
Species Reactivity NoteAll
Antibody TypeMonoclonal Antibody
Gene Symbol
  • mCherry
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~32 kDa Observed; 26.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with pFin-EF1-mCherry.

Western Blotting Analysis: 1 µg/mL of this antibody detected mCherry in 10 µg of lysate from HEK293 cells transfected with pFin-EF1-mCherry.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB131873 04054839178979

Documentation

Anti-mCherry Antibody, clone 1C51 Certificates of Analysis

TitleLot Number
Anti-mCherry, clone 1C51 3072551
Anti-mCherry, clone 1C51 - 3195413 3195413
Anti-mCherry, clone 1C51 - 3384834 3384834
Anti-mCherry, clone 1C51 - 3548329 3548329
Anti-mCherry, clone 1C51 - 3828023 3828023
Anti-mCherry, clone 1C51 - 3905522 3905522
Anti-mCherry, clone 1C51 - 4023638 4023638
Anti-mCherry, clone 1C51 - 4169252 4169252
Anti-mCherry, clone 1C51 - Q2830936A Q2830936A
Anti-mCherry, clone 1C51 Monoclonal Antibody 3128833
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