Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABS1295 Anti-mS2i6A Antibody, clone CL-3

Anti-MS2i6A, clone CL-3, Cat. No. MABS1295, is a mouse mouse monoclonal antibody that detects mS2i6A and has been tested for use in Dot Blot, ELISA, Immunocytochemistry, and Immunofluorescence.

Anti-mS2i6A Antibody, clone CL-3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling UniProt Number: N/A
View Products on Sigmaaldrich.com
MABS1295
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MaDB, ELISA, IHC, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1295
DescriptionAnti-mS2i6A Antibody, clone CL-3
Background Information2-methylthio-N6-isopentenyladenosine (mS2i6A) is a hypermodified modified nucleoside that has been shown to be present in mitochondrial tRNA. It reads codons starting with uridine. The mS2 group of mS2i6A is critical for the accurate decoding of Tyr and Phe codons. Higher concentrations of mS2i6A are found in tissues with a high energy demand, such as muscle and brain. One of the major role of mS2i6A is to stabilize the stacking interactions of the anticodon loop helix and thereby prevent codon misreading. Its deficiency has been linked to a higher rejection rate of tRNA4Leu by more aggressive proofreading on the wild-type ribosome. However, it is shown that mS2i6A has no effect on codon-anticodon interactions on wild-type ribosomes as long as aminoacyl-tRNA is in ternary complex with EF-Tu and GTP. Also, it has been reported that the lack of mS2i6A does not decrease the ability of the tRNA to be charged with its cognate amino acid, but it alters the efficiency with which the tRNA is bound to the ribosome. CDK5RAP1, which inhibits the activity of CDK5, is shown to be an N6-isopentenyladenosine methylthiotransferase. It converts the RNA modification N6-isopentenyladenosine (i6A) in mS2i6A post-synthetically. This conversion is observed both in the mitochondrial as well as nuclear RNA species. (Ref.: Wilson, RK and Roe, BA (1989). Proc. Natl. Acad. Sci. USA. 86(2); 409-413, Diaz, I., and Ehrenberg, M (1991). J. Mol. Biol. 222(4); 1161-71; Reiter, V et al. (2012). Nucleic Acids Res. 40(13); 6235-40).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MS2i6A, clone CL-3, Cat. No. MABS1295, is a mouse mouse monoclonal antibody that detects mS2i6A and has been tested for use in Dot Blot, ELISA, Immunocytochemistry, and Immunofluorescence.
Key Applications
  • Dot Blot
  • ELISA
  • Immunohistochemistry
  • Immunofluorescence
Application NotesELISA Analysis: A representative lot detected mS2i6A in ms2i6A or i6A wells (Courtesy of Fan-Yan Wei, Ph.D., Faculty of Life Sciences, Kumamoto University Japan).

Immunofluorescence Analysis: A representative lot detected mS2i6A in HeLa cells treated with mitotracker (Courtesy of Fan-Yan Wei, Ph.D., Faculty of Life Sciences, Kumamoto University Japan).

Dot Blot Analysis: A 1:200 dilution from a representative lot detected mS2i6A in Wild type (WT) total RNA and Cdk5rap1 KO mice total RNA samples from liver (Courtesy of Fan-Yan Wei, Ph.D., Faculty of Life Sciences, Kumamoto University Japan).
Biological Information
ImmunogenA synthesized mS2i6A oligonucleotide conjugated to BSA.
CloneCL-3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3 (CL-3) recognizes wild-type total RNA but not knockout total RNA.
IsotypeIgG2aκ
Species Reactivity
  • Mammals
Species Reactivity NoteMammalian cells containing mitochondria.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G purified
UniProt Number
Molecular WeightN/A
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells with mitotracker.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected mS2i6A in HeLa cells with Mitotracker.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS1295 04054839383601