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05-1564 Anti-mTOR Antibody, clone 21A12.2

Detect mTOR using this Anti-mTOR Antibody, clone 21A12.2 validated for use in WB & IC.

Anti-mTOR Antibody, clone 21A12.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: DKFZp686B11164, KIAA1999, MGC39830, mAVO3, pianissimo UniProt Number: Q6R327
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05-1564
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1564
DescriptionAnti-mTOR Antibody, clone 21A12.2
Alternate Names
  • TORC2-specific protein AVO3
  • rapamycin-insensitive companion of mTOR
Background InformationmTOR (Mammalian Target of Rapamycin, FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 consists of at least mTOR, Raptor, and GL. mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The other mTOR complex, mTORC2, is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. TORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473. TORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
References
Product Information
FormatPurified
Control
  • HEK293 cell lysates
PresentationPurified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl, with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect mTOR using this Anti-mTOR Antibody, clone 21A12.2 validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesConfocal Immunofluorescent Immunocytochemistry:
Biological Information
ImmunogenFull-length human mTOR
Cloneclone 21A12.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody only detects mTOR.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Other species to be tested.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DKFZp686B11164
  • KIAA1999
  • MGC39830
  • mAVO3
  • pianissimo
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Plays an essential role in embryonic growth and development By similarity. Part of the TORC2 complex which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.

SUBUNIT STRUCTURE: Forms part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to FRAP1 and PROTOR1 within the TORC2 complex. May interact with PROTOR2.

SEQUENCE CAUTION: The sequence CAH18135.1 differs from that shown. Reason: Frameshift at position 251.

Molecular Weight~289 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting on HEK293 cell lysates.

Western Blotting Analysis: 1:1,000 - 2,000 dilution of this antibody was used to detect mTOR in HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-1564 04053252402548

Documentation

Anti-mTOR Antibody, clone 21A12.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-mTOR Antibody, clone 21A12.2 Certificates of Analysis

TitleLot Number
Anti-mTOR, clone 21A12.2 2476749
Anti-mTOR, clone 21A12.2 - 2376018 2376018
Anti-mTOR, clone 21A12.2 - 2000824 2000824
Anti-mTOR, clone 21A12.2 - 2278558 2278558
Anti-mTOR, clone 21A12.2 - 3204243 3204243
Anti-mTOR, clone 21A12.2 - 3273620 3273620
Anti-mTOR, clone 21A12.2 - 3323746 3323746
Anti-mTOR, clone 21A12.2 - 3660411 3660411
Anti-mTOR, clone 21A12.2 - 3852337 3852337
Anti-mTOR, clone 21A12.2 - 4112558 4112558
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