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MABN1802 Anti-pan-PMCA Antibody, clone 5F10

Anti-pan-PMCA, clone 5F10, Cat. No. MABN1802, is a mouse monoclonal antibody that targets pan-Plasma membrane calcium-transporting ATPase (PMCA) and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-pan-PMCA Antibody, clone 5F10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: ATP2B1, ATP2B2, ATP2B3, ATP2B4 UniProt Number: P20020, Q01814, Q16720, P23634
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MABN1802
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, R, HELISA, ICC, IH(P), IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1802
DescriptionAnti-pan-PMCA Antibody, clone 5F10
Alternate Names
  • Plasma membrane calcium-transporting ATPase
  • Plasma membrane calcium pump
Background InformationPlasma membrane calcium-transporting ATPase (isoforms 1-4) UniProt: P20020, Q01814, Q16720, P23634; also known as PMCA, Plasma membrane calcium pump) is encoded by the ATP2B1, ATP2B2, ATP2B3, ATP2B4 genes (Gene ID: 490; 491; 492; 493) in human. PMCAs are multi-pass membrane proteins that act as a calcium/calmodulin-regulated and magnesium-dependent enzymes and catalyze the hydrolysis of ATP coupled with the transport of calcium out of the cell. PMCAs serve as the main off switch for calcium signals in all types of cells. Although there are four separate genes that code for PMCAs 1-4, alternative splicing of the primary transcripts further increases the number of PMCA variants to about 20, which differ significantly in their regulatory and kinetic properties. PMCA activity is shown to be regulated at (a) the transcriptional level (b) by calmodulin binding and (c) by their subcellular localization. The localization of different PMCAs is regulated in various complex, cell type-specific ways. PMCA1 is ubiquitously expressed, PMCA2 is mainly expressed in brain cortex, but lower levels are also found in skeletal muscle, heart muscle, stomach, liver, kidney and lung. PMCA3 is highly expressed in the cerebellum, particularly in the presynaptic terminals of parallel fibers-Purkinje neurons. PMCA4 is expressed ubiquitously, however, its tissue distribution varies with its isoforms. (Ref.: Antalffy, G et al. (2013). Biochim. Biophys. Acta 1833(12); 2561-2572; Strehler, EE (2015). Biochem. Biophys. Commun. 460 (1); 26-33)
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-pan-PMCA, clone 5F10, Cat. No. MABN1802, is a mouse monoclonal antibody that targets pan-Plasma membrane calcium-transporting ATPase (PMCA) and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected pan-PMCA in human pancreas and human kidney tissue.

Immunocytochemistry Analysis: A representative lot detected pan-PMCA in Immunocytochemistry applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Immunoprecipitation Analysis: A representative lot immunoprecipitated pan-PMCA in Immunoprecipitation applications (DeMarco, S.J., et. al. (2002) J Biol Chem. 277(12):10506-11).

Western Blotting Analysis: A representative lot detected pan-PMCA in Western Blotting applications (Paszty, K., et. al. (2005). Biochem J. 391(Pt 3):687-92; Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).

ELISA Analysis: A representative lot detected pan-PMCA in ELISA applications (Caride, A.J., et. al. (1996). Biochem J. 316 ( Pt 1):353-9).
Biological Information
ImmunogenPurified human erythrocyte calcium ATPase.
Clone5F10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5F10 detects all isoforms of Plasma membrane calcium-transporting ATPase (PMCA) in human, mouse, and rat.
IsotypeIgG2aκ
Species Reactivity
  • Chicken
  • Rat
  • Human
Species Reactivity NoteHuman, Chicken, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ATP2B1
  • ATP2B2
  • ATP2B3
  • ATP2B4
Purification MethodProtein G purified
UniProt Number
Molecular Weight134 to 139 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected pan-PMCA in human cerebral cortex tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABN1802 04054839399763