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04-736 Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal

Anti-Akt1 Antibody, clone SK703 detects level of phospho-Akt1/PKBα (Ser473) & has been published & validated for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: AKT1, RAC, PRKBA, RAC-ALPHA, PKB, MGC99656, RAC-PK-alpha, C-AKT, AKT, EC 2.7.11.1 UniProt Number: P31749
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04-736
100 µl  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-736
Replaces05-736
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal
Alternate Names
  • AKT1 kinase
  • Protein kinase B
  • RAC-alpha serine/threonine-protein kinase
  • rac protein kinase alpha
  • v-akt murine thymoma viral oncogene homolog 1
Background InformationThe serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKBα or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3). Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.
References
Product Information
FormatCulture Supernatant
Control
  • Untreated and PDGF treated NIH-3T3 fibroblasts.
PresentationCultured supernantant in 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-Akt1 Antibody, clone SK703 detects level of phospho-Akt1/PKBα (Ser473) & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenPeptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa
EpitopePhosphorylated Ser473
CloneSK703
HostRabbit
SpecificityRecognizes phosphorylated Akt1/PKBα, Mr 60 kDa. Cross-reactivity to phosphorylated Akt2 and Akt3 likely based on sequence homology.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman and mouse. Predicted cross-reactivity with rat, bovine, chicken, and Xenopus based on immunogen sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • AKT1
  • RAC
  • PRKBA
  • RAC-ALPHA
  • PKB
  • MGC99656
  • RAC-PK-alpha
  • C-AKT
  • AKT
  • EC 2.7.11.1
Modifications
  • Phosphorylation
UniProt Number
Molecular Weight60kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µl
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
04-736 04053252372315

Documentation

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone SK703, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-phospho-Akt1/PKB (Ser473), clone SK703 - 2029640 2029640
Anti-phospho-Akt1/PKB (Ser473), clone SK703 - 2073589 2073589
Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NG1752247 NG1752247
Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NG1800782 NG1800782
Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NG1848906 NG1848906
Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NG1861479 NG1861479
Anti-phospho-Akt1/PKB (Ser473), clone SK703 - NRG1685842 NRG1685842
Anti-phospho-Akt1/PKBα (Ser473), -2795414 2795414
Anti-phospho-Akt1/PKBα (Ser473), clone SK703 - 3845818 3845818
Anti-phospho-Akt1/PKBα (Ser473), clone SK703 -2585815 2585815
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies