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07-1343 Anti-phospho-B-Raf (Ser445) Antibody

Anti-phospho-B-Raf (Ser445) Antibody detects level of phospho-B-Raf (Ser445) & has been published & validated for use in WB, EA.

Anti-phospho-B-Raf (Ser445) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: G-proteins Gene Symbol: BRAF UniProt Number: P15056
07-1343
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, R, H, Xn, Ch, Ca, EqWB, Enzyme AssaysRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1343
DescriptionAnti-phospho-B-Raf (Ser445) Antibody
Alternate Names
  • B-RAF
  • B-Raf
  • BRAF1
  • p94
  • Raf
  • RAF
Background InformationThe Raf proteins (Raf-1, A-Raf, B-Raf) are Ser/Thr kinases with homology to the PKC family, containing an N-terminal regulatory domain and a C-terminal catalytic domain. Members of the Raf family bind to activated Ras GTPase, which results in Raf translocation to the plasma membrane and activation. Activated Raf proteins phosphorylate MEKs, and are therefore the principle transducers of signals from Ras to MAP kinase. B-Raf acts as a signal transducer from membrane-associated receptors to nuclear transcription factors. B-Raf is important for the regulation of cell proliferation and determination of cell fate during embryogenesis. B-Raf acts downstream of Ras and upstream of MEK in the Ras-Raf-MEK-ERK signal transduction pathway, which is a conserved RAS-activated protein kinase cascade that regulates cell growth, proliferation, and differentiation in response to growth factors, cytokines, and hormones.
References
Product Information
FormatAffinity Purified
Control
  • NIH/3T3 untreated and PDGF treated cell lysates.
PresentationPurified Rabbit Serum in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide
Applications
ApplicationAnti-phospho-B-Raf (Ser445) Antibody detects level of phospho-B-Raf (Ser445) & has been published & validated for use in WB, EA.
Key Applications
  • Western Blotting
  • Enzyme Assays
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human B-Raf phosphorylated at and around Ser445.
EpitopePhosphorylated Ser445
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityAntibody recognizes B-Raf phosphorylated at and around Ser445.
Species Reactivity
  • Mouse
  • Rat
  • Human
  • Xenopus
  • Chicken
  • Canine
  • Horse
Species Reactivity NoteReacts with mouse and rat. Predicted to react with human, xenopus, chicken, dog and horse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq]
Gene Symbol
  • BRAF
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryB-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Defects in B-Raf are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant. Defects in B-Raf are involved in a wide range of cancers and in non-Hodgkin lymphoma (NHL) [MIM:605027].

Molecular Weight84 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in NIH/3T3 untreated and PDGF treated cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected phosphorylated B-Raf (Ser445) on NIH/3T3 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-1343 04053252583773