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ABC118 Anti-phospho Beclin Antibody (Thr119)

Detect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.

Anti-phospho Beclin Antibody (Thr119) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: BECN1 UniProt Number: Q14457
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ABC118
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC118
DescriptionAnti-phospho Beclin Antibody (Thr119)
Alternate Names
  • Beclin-1
  • Coiled-coil myosin-like BCL2-interacting protein
  • Protein GT197
Background InformationThe Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2. Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia. It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport.
References
Product Information
FormatAffinity Purified
Control
  • Human prostate tumor tissue lysate.
PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Beclin using this rabbit polyclonal antibody, Anti-phospho Beclin Antibody (Thr119) validated for use in western blotting & Peptide Inhibition Assay.
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesWestern Blotting/Peptide Inhibition Analysis: 5 µg/mL from a representative lot detected phospho Beclin (Thr119) in LNCap cell lysate. Peptide inhibition was observed with the addition of phospho peptides, but not with the addition of non-phospho peptides.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the BH3 domain of human Beclin phosphorylated at Thr119.
EpitopeBH3 domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the BH3 domain of Beclin phosphorylated at Thr119.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, Chicken, Porcine, Xenopus based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • BECN1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~50 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human prostate tumor tissue lysate.

Western Blotting Analysis: 5 µg/mL of this antibody detected 10 µg of human prostate tumor tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABC118 04053252909290

Documentation

Anti-phospho Beclin Antibody (Thr119) SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho Beclin Antibody (Thr119) Certificates of Analysis

TitleLot Number
Anti-phospho Beclin (Thr119) - 2836178 2836178
Anti-phospho Beclin (Thr119) - 3180445 3180445
Anti-phospho Beclin (Thr119) - 3454399 3454399
Anti-phospho Beclin (Thr119) - 3524337 3524337
Anti-phospho Beclin (Thr119) - 3855747 3855747
Anti-phospho Beclin (Thr119) - 4078587 4078587
Anti-phospho Beclin (Thr119) - 4215024 4215024
Anti-phospho Beclin (Thr119) - Q2196317 Q2196317
Anti-phospho Beclin (Thr119) -2527587 2527587
Anti-phospho Beclin (Thr119) -2742257 2742257
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