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06-519-AF488 Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor® 488 conjugate

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor® 488 conjugate is an antibody against phospho-CREB for use in Immunofluorescence.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: CREB1 UniProt Number: P16220
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06-519-AF488
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RIFRbAlexaFluor®647Polyclonal Antibody
Description
Catalogue Number06-519-AF488
DescriptionAnti-phospho-CREB (Ser133) Antibody, Alexa Fluor® 488 conjugate
Alternate Names
  • Cyclic AMP-responsive element-binding protein 1 (pSer133)
  • cAMP-responsive element-binding protein 1 (pSer133)
  • CREB-1 (pSer133)
  • CREB-A (pSer133)
  • htCREB (pSer133)
  • CREB-B (pSer119)
Background InformationCyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.
References
Product Information
FormatAlexaFluor®647
PresentationPurified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-CREB (Ser133) Antibody, Alexa Fluor® 488 conjugate is an antibody against phospho-CREB for use in Immunofluorescence.
Key Applications
  • Immunofluorescence
Application NotesThe unconjugated antibody (Cat. No. 06-519) is shown to be suitable also for Electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry, and Western blotting applications.
Biological Information
ImmunogenKLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB-A.
EpitopepSer133.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityRecognizes phosphorylated CREB-A (pSer133), htCREB (pSer133), and CREB-B (pSer119) spliced isoforms reported by (UniProt P16220). May also recognize phosphorylated CREM and ATF-1 that contain homologous sequence to that of the immungen peptide.
IsotypeIgG1
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Bovine, Canine, Porcine, Xenopus, Chicken, Guinea Pig based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CREB1
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~43 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunofluorescence in rat brain tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected phospho-CREB (Ser133) in rat brain tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
06-519-AF488 04055977331820

Documentation

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor® 488 conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor® 488 conjugate Certificates of Analysis

TitleLot Number
Anti-phospho-CREB (Ser133), -Q2662540 Q2662540
Anti-phospho-CREB (Ser133), Alexa Fluor® 488 conjugate - 3182597 3182597
Anti-phospho-CREB (Ser133), Alexa Fluor® 488 conjugate - 3252099 3252099
Anti-phospho-CREB (Ser133), Alexa Fluor® 488 conjugate - 3388644 3388644
Anti-phospho-CREB (Ser133), Alexa Fluor® 488 conjugate - 3429602 3429602
Anti-phospho-CREB (Ser133), Alexa Fluor® 488 conjugate - 4093165 4093165
Anti-phospho-CREB (Ser133), Alexa Fluor® 488 conjugate Polyclonal Antibody 2925943

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies