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04-281 Anti-phospho-EGFR (Thr669) Antibody, clone 5F10

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: EGFR, ERBB1, ERBB, mENA UniProt Number: P00533
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04-281
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue Number04-281
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-EGFR (Thr669) Antibody, clone 5F10
Alternate Names
  • ERBB
  • ERBB1
  • epidermal growth factor receptor
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Includes PMA and pervanadate treated A431 cell lysate as a positive control
Presentation100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.
Clone5F10
HostMouse
SpecificityRecognizes EGFR when phosphorylated at Threonine 669.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EGFR
  • ERBB1
  • ERBB
  • mENA
Modifications
  • Phosphorylation
Purification MethodThiophilic adsoption and size exclusion chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
Molecular Weight180 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 6 months at -20°C from date of shipment
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
04-281 04053252744242

Documentation

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 Certificates of Analysis

TitleLot Number
Anti-phospho-EGFR (Thr669), clone 5F10 (Mouse monoclonal IgG1) 3118673
Anti-phospho-EGFR (Thr669), clone 5F10 (Mouse monoclonal IgG1) - 2195988 2195988
Anti-phospho-EGFR (Thr669), clone 5F10 - 0191S0201 0191S0201
Anti-phospho-EGFR (Thr669), clone 5F10 - 2029610 2029610
Anti-phospho-EGFR (Thr669), clone 5F10 - 2058318 2058318
Anti-phospho-EGFR (Thr669), clone 5F10 - 3601699 3601699
Anti-phospho-EGFR (Thr669), clone 5F10 - 4144899 4144899
Anti-phospho-EGFR (Thr669), clone 5F10 - 4177425 4177425
Anti-phospho-EGFR (Thr669), clone 5F10 - DAM1484509 DAM1484509
Anti-phospho-EGFR (Thr669), clone 5F10 - NG1895478 NG1895478

References

Reference overviewPub Med ID
Cisplatin induces PKB/Akt activation and p38(MAPK) phosphorylation of the EGF receptor
Winograd-Katz, S E and Levitzki, A
Oncogene, 25:7381-90 (2006)  2006

16785992 16785992
Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor
Heisermann, G J, et al
J Biol Chem, 265:12820-7 (1990)  1990

2115882 2115882
Epidermal growth factor receptor threonine and serine residues phosphorylated in vivo
Heisermann, G J and Gill, G N
J Biol Chem, 263:13152-8 (1988)  1988

3138233 3138233

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