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ABE2945-25UL Anti-phospho EZH2 (Thr345)

Anti-phospho EZH2 (Thr345), Cat. No. ABE2945, is a rabbit polyclonal antibody that detects EZH2 phosphorylated on threonine 345 and is tested for use in Immunocytochemistry and Western Blotting.

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ABE2945-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberABE2945-25UL
DescriptionAnti-phospho EZH2 (Thr345)
Alternate Names
  • Histone-lysine N-methyltransferase EZH2
  • EC:2.1.1.356
  • ENX-1
  • Enhancer of zeste homolog 2
  • Lysine N-methyltransferase 6
Background InformationHistone-lysine N-methyltransferase EZH2 (UniProt: Q15910; also known as EC:2.1.1.356, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, EZH2) is encoded by the EZH2 (also known as KMT6) gene (Gene ID: 2146) in human. EZH2 is a polycomb group protein that is expressed in many tissues. It is the catalytic subunit of the PRC2/EED-EZH2 complex that methylates lysine 9 (H3K9me) and lysine 27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. It is shown to mono-, di- and trimethylate lysine 27 of histone H3 (H3K27me1, H3K27me2 and H3K27me3). It displays a preference for substrates with less methylation and loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. EZH2 phosphorylation by Akt1 reduces its methyltransferase activity. Its phosphorylation at threonine 345 by CDK1 and CDK2 is shown to promote maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. It is reported to regulate the circadian clock via histone methylation at the promoter of the circadian genes and is essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer. Overexpression of EZH2 has been reported in numerous tumors, including carcinomas of the breast, colon, larynx, testis, and lymphoma. Mutations in EZH2 gene are known to cause Weaver syndrome, a disease characterized by accelerated growth and osseous maturation and unusual craniofacial appearance. (Ref.: Chen, S., et al. (2010). Nat. Cell Biol. 12(11); 1108-1114; Etchegaray, J-P., et al. (2006). J. Biol. Chem. 281(30); 21209-21215; Cha, T-L., et al. (2005). Science. 310(5746); 306-310).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ200
Applications
ApplicationAnti-phospho EZH2 (Thr345), Cat. No. ABE2945, is a rabbit polyclonal antibody that detects EZH2 phosphorylated on threonine 345 and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesTested applications

Immunocytochemistry Analysis: A representative lot detected phospho EZH2 (Thr345) in Immunocytochemistry applications (Ding, L., et al. (2014). Cancer Res. 74(7); 2050-2061).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids surrounding phosphothreonine 345 from the internal region of human Histone-lysine N-methyltransferase EZH2.
EpitopeInternal
Concentration0.1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Histone-lysine N-methyltransferase EZH2 phosphorylated on threonine 345.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Frog, Mouse, Canine, Bovine, Monkey, Feline based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EZH2
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~85 kDa observed; 85.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected EZH2 phosphorylated on threonine 345 in LNCaP cell lysate, but not in lysates treated with protein phosphatase (400 units for 30 min at 30°C).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE2945-25UL 04065268979006