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ABT135 Sigma-AldrichAnti-phospho-FAK (Tyr397) Antibody

Detect phospho-FAK (Tyr397) using this Anti-phospho-FAK (Tyr397) Antibody validated for use in WB, Cell Function Assay.

Anti-phospho-FAK (Tyr397) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: PTK2, FAK, FAK1 UniProt Number: Q05397

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, H, Xn, Ch, M	WB, Cell Function Assay	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABT135
Replaces	04-974
Description	Anti-phospho-FAK (Tyr397) Antibody
Alternate Names	Focal adhesion kinase 1 FADK 1 Protein-tyrosine kinase 2 pp125FAK
Background Information	Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth. Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.

References

Product Information
Format	Affinity Purified
HS Code	3002 15 90
Control	untransfected and Src-transfected RAT2 cell lysates
Presentation	Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Detect phospho-FAK (Tyr397) using this Anti-phospho-FAK (Tyr397) Antibody validated for use in WB, Cell Function Assay.
Key Applications	Western Blotting Cell Function Assay
Application Notes	Peptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot peptide blocked on RAT2 cells transfected with Src.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human FAK phosphorylated at Tyr397.
Epitope	Phosphorylated Tyr397
Host	Rabbit
Specificity	This antibody recognizes FAK phosphorylated at Tyr397.
Species Reactivity	Rat Human Xenopus Chicken Mouse
Species Reactivity Note	Demonstrated to react with Rat. Predicted to react with Human, Xenopus, Chicken, and Mouse based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_722560
Entrez Gene Summary	This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq].
Gene Symbol	PTK2 FAK FAK1
Modifications	Phosphorylation
Purification Method	Affinity Purfied
UniProt Number	Q05397
UniProt Summary	FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB By similarity. Interacts with TGFB1I1. SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions. TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. DOMAIN: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL By similarity. The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. PTM: Phosphorylated on 6 tyrosine residues upon activation. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain.
Molecular Weight	~125 kDa observed. An uncharacterized band appears at ~190 kDa in some lysates.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in untransfected and Src-transfected RAT2 cell lysates. Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-FAK (Tyr397) in 10 µg of untransfected and Src-transfected RAT2 cell lysates.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
ABT135	04053252420603

Documentation

Anti-phospho-FAK (Tyr397) Antibody SDS

Title
Safety Data Sheet (SDS)

Anti-phospho-FAK (Tyr397) Antibody Certificates of Analysis

Title	Lot Number
Anti-phospho-FAK (Tyr397) - 2327489	2327489
Anti-phospho-FAK (Tyr397) - 2397165	2397165
Anti-phospho-FAK (Tyr397) - 2017298	2017298
Anti-phospho-FAK (Tyr397) - 2110560	2110560
Anti-phospho-FAK (Tyr397) - 2227179	2227179
Anti-phospho-FAK (Tyr397) - 2298444	2298444
Anti-phospho-FAK (Tyr397) - 2366604	2366604
Anti-phospho-FAK (Tyr397) - 3259073	3259073
Anti-phospho-FAK (Tyr397) - 3506604	3506604
Anti-phospho-FAK (Tyr397) - 3866325	3866325

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