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06-570-AF555 Anti-phospho-H3 Antibody (Ser10), Alexa Fluor® 555 Conjugate

Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate is a purified rabbit polyclonal Antibody that detects Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 555 and validated for use in IC. The unconjugated version of this antibody is published & proven in ICC, IP & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, HIST3H3, H3FT, H3T, MGC126886 UniProt Number: Q16695
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06-570-AF555
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCRbAlexaFluor®555Polyclonal Antibody
Description
Catalogue Number06-570-AF555
DescriptionAnti-phospho-H3 Antibody (Ser10), Alexa Fluor® 555 Conjugate
Alternate Names
  • H3S10P
  • Histone H3 (phospho Ser10)
  • H3 histone, family 3A
  • H3.3A
  • H3 histone, family 3B
  • H3.3B
Background InformationHistone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.
References
Product Information
FormatAlexaFluor®555
PresentationPurified rabbit polyclonal in buffer containing PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate is a purified rabbit polyclonal Antibody that detects Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 555 and validated for use in IC. The unconjugated version of this antibody is published & proven in ICC, IP & WB.
Key Applications
  • Immunocytochemistry
Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot of the non-conjugated form of this antibody (Cat. # 06-570) immunoprecipitated phospho-Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.

Beadlyte® Assay Analysis: A representative lot of the non-conjugated form of this antibody (Cat. # 06-570) recognized Histone H3 phosphorylated on Ser10 by Luminex assay.

Western Blot Analysis: 0.5-1 µg/mL from a representative lot of the non-conjugated form of this antibody (Cat. # 06-570) detected phospho-Histone H3 (Ser10) on 10 µg of Colcemid-treated HeLa acid extract, but not on untreated HeLa acid extract.
Biological Information
ImmunogenLinear peptide corresponding to phospho-Histone H3 (Ser10).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
  • HIST3H3
  • H3FT
  • H3T
  • MGC126886
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
06-570-AF555 04055977115819

Documentation

Anti-phospho-H3 Antibody (Ser10), Alexa Fluor® 555 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-H3 Antibody (Ser10), Alexa Fluor® 555 Conjugate Certificates of Analysis

TitleLot Number
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate Q2483345
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate 2813459
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate - 3206320 3206320
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate - 3336225 3336225
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate - 3884343 3884343
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate - 3980762 3980762
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate -2888815 2888815
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate Polyclonal Antibody 2968688
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate Polyclonal Antibody 3115818
Anti-phospho-H3 (Ser10), Alexa Fluor® 555 Conjugate Polyclonal Antibody 2884267