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05-806-AF488 Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 488

This Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 488 is validated for use in Immunocytochemistry for the detection of phospho-Histone H3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3T, H3FT, HIST3H3, H3FJ, HIST1H3J, H3FF, HIST1H3I, H3FK, HIST1H3H, H3FH, HIST1H3G, H3FI, HIST1H3F, H3FD, HIST1H3E, H3FB, HIST1H3D, H3FC, HIST1H3C, H3FL, HIST1H3B, H3FA
 UniProt Number: Q16695
05-806-AF488
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-806-AF488
DescriptionAnti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 488
Alternate Names
  • H3S10P
  • Histone H3, Ser10 phosphorylated
  • H3 histone, family 3A, Ser10 phosphorylated
  • H3 histone, family 3B, Ser10 phosphorylated
  • H3.3B, Ser10 phosphorylated
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 488 is validated for use in Immunocytochemistry for the detection of phospho-Histone H3.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected histone H3 Ser10 phosphorylation in A431 cells.
The unconjugated antibody (Cat. No. 05-806) is shown to be suitable also for flow cytometry, multiplex assay, chromatin Western blotting, immunogen peptide inhibition assay, immunofluorescence and immunocytochemistry applications.
Biological Information
ImmunogenA proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3.
Epitope Histone H3 pSer10.
Clone3H10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityHistone H3 phosphorylated at Ser10.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Broad species cross-reactivity is expected.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3T
  • H3FT
  • HIST3H3
  • H3FJ
  • HIST1H3J
  • H3FF
  • HIST1H3I
  • H3FK
  • HIST1H3H
  • H3FH
  • HIST1H3G
  • H3FI
  • HIST1H3F
  • H3FD
  • HIST1H3E
  • H3FB
  • HIST1H3D
  • H3FC
  • HIST1H3C
  • H3FL
  • HIST1H3B
  • H3FA
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~17 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H3 Ser10 phosphorylation in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-806-AF488 04055977314632