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05-806-AF555 Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 555

Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 555 is an antibody against phospho-Histone H3 for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
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05-806-AF555
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®555Monoclonal Antibody
Description
Catalogue Number05-806-AF555
DescriptionAnti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 555
Alternate Names
  • H3S10P
  • Histone H3 (phospho Ser10)
  • H3 histone, family 3A
  • H3.3A
  • H3 histone, family 3B
  • H3.3B
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
FormatAlexaFluor®555
PresentationPurified mouse monoclonal IgG1κ antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 555 is an antibody against phospho-Histone H3 for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H3 (Ser10) in HepG2 cells.
The unconjugated antibody (Cat. No. 05-806) is shown to be suitable also for peptide inhibition assay (PIA), flow cytometry, immunocytochemistry, immunofluorescence, multiplex assay, Western blotting applications.
Biological Information
ImmunogenA proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3.
EpitopeH3 pSer10.
Clone3H10
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3H10 recoginzes histone N-terminal peptide with Ser10 phosphorylation (pSer10) by bead-based peptide specificity assay. Clone 3H10 does not bind unphosphorylated peptides or peptides with only pThr11 or pSer28 modification.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Broad species cross-reactivity expected.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~17 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-806-AF555 04055977311938

Documentation

Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 555 SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor® 555 Certificates of Analysis

TitleLot Number
Anti-phospho-Histone H3 (Ser10), clone 3H10, Alexa Fluor® 555 - 3706638 3706638
Anti-phospho-Histone H3 (Ser10), clone 3H10, Alexa Fluor® 555 - 4005667 4005667
Anti-phospho-Histone H3 (Ser10), clone 3H10, Alexa Fluor® 555 - 4127500 4127500
Anti-phospho-Histone H3 (Ser10), clone 3H10, Alexa Fluor® 555 -Q2637235 Q2637235
Anti-phospho-Histone H3 (Ser10), clone 3H10, Alexa Fluor® 555 Monoclonal Antibody 3071898