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17-10400 Anti-phospho-MLKL (Ser358) Antibody Set

Anti-phospho-MLKL (Ser358) Antibody Set is an antibody set against phospho-MLKL (Ser358) for use in WB.

Anti-phospho-MLKL (Ser358) Antibody Set MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: MLKL UniProt Number: Q8NB16
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17-10400
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Description
Catalogue Number17-10400
DescriptionAnti-phospho-MLKL (Ser358) Antibody Set
OverviewThis phospho-MLKL (Ser358) antibody set contains one vial of phospho-MLKL (Ser358) antibody and one vial of MLKL “Total” antibody.

RECOMMENDED USAGE:
It is recommended to first perform immunoprecipitation on your sample using the non-phosphorylated MLKL antibody for “total” MLKL protein isolation (see protocol section on page 2 of the Certificate of Analysis); then use the immunoprecipitated sample to detect the phosphorylated protein using the phospho-MLKL (Ser358) antibody via western blot.
Alternate Names
  • Mixed lineage kinase domain-like protein
  • MLKL
Background InformationMixed lineage kinase domain-like protein (MLKL) is required for the execution of programmed necrosis. MLKL is a cell-permeable acrylamide compound that inhibits human, but not murine, MLKL adaptor function via covalent modification of Cys86. MLKL prevents the MLKL-RIP1-RIP3 necrosome complex from interacting with further downstream effectors.
References
Product Information
Components
  • One vial of phospho-MLKL (Ser358) antibody
  • One vial of MLKL “Total” antibody
FormatAffinity Purified
Presentation100 µL of Anti-phospho-MLKL (Ser358) purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

100 µg (concentration: 1 mg/mL) of Anti- MLKL purified polyclonal antibody in 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-MLKL (Ser358) Antibody Set is an antibody set against phospho-MLKL (Ser358) for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis:
Untreated HT-29 cell lysates or lysates from HT-29 cells treated with Cycloheximide,Z-VAD-FMK and TNF alpha were first immunoprecipitated using the Anti-MLKL “total” antibody (1:1000 dilution) to isolate the MLKL protein.
The isolated “total” MLKL protein sample was then probed with Anti-phospho-MLKL (Ser358) (1:1000 dilution).
Biological Information
ImmunogenLinear peptide corresponding to the Protein Kinase Domain of phospho-MLKL (Ser358).
EpitopeSer358
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityWhen used together and under the “Recommended Usage” instructions, this antibody set will detect MLKL phosphorylated at Ser358.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Gene Symbol
  • MLKL
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Required for the execution of programmed necrosis.

SUBUNIT STRUCTURE: Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3. Within this complex, may play a role in the proper targeting of RIPK1/RIPK3 to its downstream effector PGAM5.

DOMAIN: The protein kinase domain is predicted to be catalytically inactive.

SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Contains 1 protein kinase domain.
Molecular Weight~54 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceAnti- MLKL (Total Antibody): Evaluated by Immunoprecipitation on HT-29 cell lysates.

Anti-phospho-MLKL (Ser358) Antibody: Evaluated by Dot Blot on phosphorylated and non-phosphorylated MLKL peptides.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size1 Set
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
17-10400 04053252934599

Documentation

Anti-phospho-MLKL (Ser358) Antibody Set SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-MLKL (Ser358) Antibody Set Certificates of Analysis

TitleLot Number
Anti-phospho-MLKL (Ser358) Antibody Set - 2398922 2398922
Anti-phospho-MLKL (Ser358) Antibody Set - 2449234 2449234
Anti-phospho-MLKL (Ser358) -2569813 2569813
Anti-phospho-MLKL (Ser358) -2578114 2578114
Anti-phospho-MLKL (Ser358) -2593176 2593176
Anti-phospho-MLKL (Ser358) -2599377 2599377
Anti-phospho-MLKL (Ser358) -2662282 2662282
Anti-phospho-MLKL (Ser358) -2762210 2762210
Anti-phospho-MLKL (Ser358) -2784344 2784344
Anti-phospho-MLKL (Ser358) -2785887 2785887
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