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04-773 Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal

Anti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: MBS, MGC133042, MYPT1 UniProt Number: O14974
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04-773
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Ch, H, M, RWBRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-773
Replaces05-773
DescriptionAnti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal
Alternate Names
  • Myosin phosphatase target subunit 1
  • Myosin phosphatase-targeting subunit 1
  • Protein phosphatase myosin-binding subunit
  • myosin phosphatase, target subunit 1
  • protein phosphatase 1, regulatory (inhibitor) subunit 12A
Background InformationPhosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.
References
Product Information
FormatCulture Supernatant
HS Code3002 15 90
Control
  • HEK293 cell lysate phosphorylated by ROKα/ROCKII.
PresentationCultured supernantant containing 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.
EpitopepThr850(Ch) or pThr853(H)
CloneSA19
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.
IsotypeIgG
Species Reactivity
  • Chicken
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Chicken.
Predicted to cross-react with mouse and rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryMyosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine
triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase.
Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased
phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin
phosphatase through the action of Rho-kinase. Several transcript
variants encoding different isoforms have been found for this gene.
Gene Symbol
  • MBS
  • MGC133042
  • MYPT1
Modifications
  • Phosphorylation
UniProt Number
UniProt SummaryFUNCTION: Regulates myosin phosphatase activity.
SUBUNIT STRUCTURE: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT By similarity. Binds PPP1R12B, ROCK1 and IL16.
SUBCELLULAR LOCATION: Cytoplasm. Note: Along actomyosin filaments and stress fibers. Ref.8
PTM: Phosphorylated by CIT (Rho-associated kinase) By similarity. Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Contains 6 ANK repeats.
Molecular WeightDoublet at ~130 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceWesterin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µl
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
04-773 04053252326356

Documentation

Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-phospho-MYPT1 (Thr850), clone SA19 -
Anti-phospho-MYPT1 (Thr850), clone SA19 - NG1915317 NG1915317
Anti-phospho-MYPT1 (Thr850), -2578047 2578047
Anti-phospho-MYPT1 (Thr850), -2585817 2585817
Anti-phospho-MYPT1 (Thr850), -2593155 2593155
Anti-phospho-MYPT1 (Thr850), -2700778 2700778
Anti-phospho-MYPT1 (Thr850), -2779438 2779438
Anti-phospho-MYPT1 (Thr850), clone SA19 - 2010086 2010086
Anti-phospho-MYPT1 (Thr850), clone SA19 - 2091729 2091729
Anti-phospho-MYPT1 (Thr850), clone SA19 - 2436428 2436428
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