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07-868 Sigma-AldrichAnti-phospho-PKA Catalytic β subunit (Ser338) Antibody

This Anti-phospho-PKA Catalytic β subunit (Ser338) Antibody is validated for use in WB for the detection of phospho-PKA Catalytic β subunit (Ser338).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PRKACB, PKACB, MGC41879, MGC9320, DKFZp781I2452 UniProt Number: P22694

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Key Specifications Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	07-868
Brand Family	Upstate
Trade Name	Upstate
Description	Anti-phospho-PKA Catalytic β subunit (Ser338) Antibody

References

Product Information
Format	Affinity Purified
Presentation	100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Quality Level	MQ100

Applications
Application	This Anti-phospho-PKA Catalytic β subunit (Ser338) Antibody is validated for use in WB for the detection of phospho-PKA Catalytic β subunit (Ser338).
Key Applications	Western Blotting

Biological Information
Immunogen	Peptide corresponding to amino acid region encompassing the human, cow, and pig phospho-PKA Catalytic β (Ser338).
Host	Rabbit
Specificity	Recognizes phospho-PKA Catalytic β subunit (Ser338).
Isotype	IgG
Species Reactivity	Mouse
Species Reactivity Note	Predicted to cross-react with human based on sequence homology (100% homologous).
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_002731.2 NM_182948.2 NM_207578.1
Entrez Gene Summary	cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene Symbol	PRKACB PKACB MGC41879 MGC9320 DKFZp781I2452
Modifications	Phosphorylation
Purification Method	ImmunoAffinity Purified
UniProt Number	P22694
UniProt Summary	FUNCTION: SwissProt: P22694 # Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. COFACTOR: Magnesium. SIZE: 351 amino acids; 40623 Da SUBUNIT: A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity). TISSUE SPECIFICITY: Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoforms 3 and 4 are only epxressed in the brain. PTM: Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively (By similarity). SIMILARITY: SwissProt: P22694 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
Molecular Weight	~40 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by immunoblot.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 2 years at -20°C from date of shipment. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
07-868	04053252513978

Documentation

Anti-phospho-PKA Catalytic β subunit (Ser338) Antibody SDS

Title
Safety Data Sheet (SDS)

Anti-phospho-PKA Catalytic β subunit (Ser338) Antibody Certificates of Analysis

Title	Lot Number
Anti-phospho-PKA Catalytic b subunit (Ser338) (Affinity purified Rabbit polyclonal IgG) - 2015531	2015531
Anti-phospho-PKA Catalytic b subunit (Ser338) (Affinity purified Rabbit polyclonal IgG) - 2273802	2273802
Anti-phospho-PKA Catalytic b subunit (Ser338) - DAM1487535	DAM1487535
Anti-phospho-PKA Catalytic b subunit (Ser338) - DAM1588196	DAM1588196
Anti-phospho-PKA Catalytic b subunit (Ser338) - NG1900703	NG1900703
Anti-phospho-PKA Catalytic β subunit (Ser338) (Affinity purified Rabbit polyclonal IgG)	2983454
Anti-phospho-PKA Catalytic β subunit (Ser338) - 2530513	2530513
Anti-phospho-PKA Catalytic β subunit (Ser338) - 3448832	3448832
Anti-phospho-PKA Catalytic β subunit (Ser338) - 3904625	3904625
Anti-phospho-PKA Catalytic β subunit (Ser338) - 3914215	3914215

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