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ABS2246-100UG Anti-phospho PKLR (Ser113)

Anti-phospho PKLR (Ser113), Cat. No. ABS2246, is a rabbit polyclonal antibody that detects Pyruvate kinase PKLR phosphorylated on serine 113 and is tested for use in Immunofluorescence and Western Blotting.

ABS2246-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABS2246-100UG
DescriptionAnti-phospho PKLR (Ser113)
Alternate Names
  • Pyruvate kinase PKLR
  • EC:2.7.1.40
  • L-PK
  • Pyruvate kinase isozymes L
Background InformationPyruvate kinase PKLR (UniProt: P12928-2; also known as EC:2.7.1.40, L-PK, Pyruvate kinase isozymes L/R) is encoded by the Pklr gene (Gene ID: 24651) in rat. Pyruvate kinas (PK) is a key glycolytic enzyme that catalyzes the hydrolysis of phosphoenolpyruvate (PEP) to pyruvate, generating ATP in this process. Four tissue specific isozymes of PK have been described in mammals: L (liver), R (erythrocytes), M1 (muscle) and ubiquitous M2. Except for M1 isozyme all other isozymes display positive cooperative kinetic behavior upon binding the allosteric activator fructose-1,6-diphosphate and in the presence of increasing concentrations of PEP. Phosphorylation of PK liver isozyme at serine 113 has been correlated with insulin resistance in rats fed a high-fat diet. Mice fed a high fat diet exhibit a significantly increased level of phospho-Ser 113 form, which declines significantly upon treatment with a pyruvate kinase activator. It has been shown that in rats fed a high-fat diet CDKs are activated and cause phosphorylation of serine 113. The amino acid sequence surrounding pS113 site is shown to have features of a CDK substrate. Phosphorylation of liver PK at serine 113 results in its retention in the nucleus and inhibition of CDK can reduce its nuclear retention and cause an increase in cytosolic enzyme activity. (Ref.: Abulizi, A., et al. (2020). Cell Metab. 32(5); 751-766; Gassaway, BM., et al. (2019). Cell Rep. 29(11); 3394-3404).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-phospho PKLR (Ser113), Cat. No. ABS2246, is a rabbit polyclonal antibody that detects Pyruvate kinase PKLR phosphorylated on serine 113 and is tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesWestern Blotting Analysis (WB): A representative lot of this antibody detected Full-length phosphorylated PKL pS113 in Western Blotting applications. (Courtesy of Dr. Jesse Rinehart Lab, Yale University).

Western Blotting Analysis: A representative lot detected Pyruvate kinase PKLR in Western Blotting applications. (Gassaway, B.M., et.al, (2019). Cell Rep. 29(11):3394-3404).

Immunofluorescence Analysis: A representative lot detected Pyruvate kinase PKLR in Immunofluorescence applications (Gassaway, B.M., et.al, (2019). Cell Rep. 29(11):3394-3404).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids surrounding the phosphoserine 113 from the N-terminal region of rat Pyruvate. kinase PKLR.
EpitopeN-terminal
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Pyruvate Kinase PKLR phosphorylated on serine 113.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteRat. Predicted to react with Human, Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Pklr
Modifications
  • Phosphorylation
Purification MethodProtein A purified
UniProt Number
Molecular Weight~58 kDa observed; 58.79 kDa calculated for L-isoform. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting on Full-length phosphorylated PKL pS113.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected full-length rat Pyruvate kinase (PKLR) phosphorylated on serine 113, but not the unphosphorylated PKLR
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABS2246-100UG 04065265324380