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04-795 Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27, rabbit monoclonal

Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27 is an antibody against phospho-Ribosomal Protein S6 (Ser235) for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling, Apoptosis & Cancer Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: 40S ribosomal protein S6, Phosphoprotein NP33, ribosomal protein S6, RPS6 UniProt Number: P62753
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-795
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27, rabbit monoclonal
Alternate Names
  • 40S ribosomal protein S6
  • Phosphoprotein NP33
  • ribosomal protein S6
Background InformationS6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that is known to be phosphorylated by both p70S6 Kinase as well as RSK as well as Ser240/244 which is thought to only be phosphorylated by p70S6K.
References
Product Information
FormatCulture Supernatant
Control
  • Serum stimulated 3T3 cell lysate.
PresentationCultured supernatant with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27 is an antibody against phospho-Ribosomal Protein S6 (Ser235) for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis:
A 1:500-1:2,000 dilution of this lot detected phosphorylated ribosomal protein S6 in lysates from serum stimulated 3T3 cells.
Biological Information
ImmunogenSynthetic peptide containing the sequence …RRL[pS]SL… in which pS corresponds to phospho-serine at residue 235 of human ribosomal protein S6. The immunizing sequence is identical in mouse, rat, chicken and Xenopus.
EpitopePhosphorylated Ser235
CloneMC27
HostRabbit
SpecificityRecognizes ribosomal protein S6 only when phosphorylated on Ser235.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, rat and mouse. Broad species cross-reactivity predicted, due to sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryRibosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]
Gene Symbol
  • 40S ribosomal protein S6
  • Phosphoprotein NP33
  • ribosomal protein S6
  • RPS6
Modifications
  • Phosphorylation
UniProt Number
UniProt SummaryFUNCTION:May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

PTM: Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

SIMILARITIES: Belongs to the ribosomal protein S6e family.
Molecular Weight~30 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot on serum stimulated 3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
04-795 04053252512582

Documentation

Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27, rabbit monoclonal SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Ribosomal Protein S6 (Ser235) Antibody, clone MC27, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-phospho-Ribosomal Protein S6 (Ser235), clone MC27 - 2462814 2462814
Anti-phospho-Ribosomal Protein S6 (Ser235), clone MC27 - DAM1561704 DAM1561704
Anti-phospho-Ribosomal Protein S6 (Ser235), clone MC27 -2608737 2608737
Anti-phospho-Ribosomal Protein S6 (Ser235), clone MC27 -2854620 2854620
Anti-phospho-Ribosomal Protein S6 (Ser235), clone MC27 Monoclonal Antibody 3060085
Anti-phospho-Ribosomal Protein S6 -2752542 2752542