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ABS1849 Anti-phospho-Rsk1 (Thr359/Ser363) Antibody

Anti-Rsk1 Antibody is an antibody against phospho-Rsk1 (Thr359/Ser363) for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Peptide Inhibition Assay.

Anti-phospho-Rsk1 (Thr359/Ser363) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: RPS6KA1, MAPKAPK1A, RSK1 UniProt Number: Q15418
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ABS1849
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, IHC, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1849
DescriptionAnti-phospho-Rsk1 (Thr359/Ser363) Antibody
Alternate Names
  • Ribosomal protein S6 kinase alpha-1, Thr359/Ser363 phosphorylated
  • 90 kDa ribosomal protein S6 kinase 1, Thr359/Ser363 phosphorylated
  • MAP kinase-activated protein kinase 1a, Thr359/Ser363 phosphorylated
  • MAPK-activated protein kinase 1a, Thr359/Ser363 phosphorylated
  • MAPKAP kinase 1a, Thr359/Ser363 phosphorylated
  • MAPKAPK-1a, Thr359/Ser363 phosphorylated
  • p90-RSK 1, Thr359/Ser363 phosphorylated
  • p90RSK1, Thr359/Ser363 phosphorylated
  • p90S6K, Thr359/Ser363 phosphorylated
  • Ribosomal S6 kinase 1, Thr359/Ser363 phosphorylated
  • RSK-1, Thr359/Ser363 phosphorylated
  • S6K-alpha-1, Thr359/Ser363 phosphorylated
Background InformationRibosomal protein S6 kinase alpha-1 (EC 2.7.11.1; UniProt Q15418; also known as 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, p90-RSK 1, p90RSK1, p90S6K, Ribosomal S6 kinase 1, RSK-1, S6K-alpha-1) is encoded by the RPS6KA1 (also known as MAPKAPK1A, RSK1) gene (Gene ID 6195) in human. Ribosomal s6 kinases (RSKs) are serine/threonine kinases that play a role in mediating Mek1/2-Erk1/2 mitogen-activated protein (MAP) kinase pathway signaling events. There exist two types of RSKs, p70rsk (a.k.a. S6 Kinase or S6-H1 kinase) and p90rsk (a.k.a. MAPK-activated protein kinase). Six p90rsk-coding genes have been identified in human, RPS6KA1 (MAPKAPK1A, RSK1), RPS6KA2 (MAPKAPK1C, RSK3), RPS6KA3 (MAPKAPK1B, RSK2), RPS6KA4 (MSK2), RPS6KA5 (MSK1), and RPS6KA6 (RSK4). Erk1/2 activates p90RSK1 by sequential phosphorylation events, where it first phosphorylates Rsk1 C-terminal kinase domain (CTKD) Thr573, leading to subsequent Ser380 phosphorylation in the N-terminal AGC kinase-type domain. Alternatively, Ser380 can be phosphorylated directly by MK2/3 independent of CTKD.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Rsk1 Antibody is an antibody against phospho-Rsk1 (Thr359/Ser363) for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Peptide Inhibition Assay.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry
  • Peptide Inhibition Assay
Application NotesImmunoprecipitation Analysis: 4.5 µg from a representative lot immunoprecipitated Thr359/Ser363-phosphorylated Rsk1 from 500 µg of lysate from EGF-treated A431 cells.
Immunohistochemistry Analysis: A 1:250-1000 dilution from a representative lot detected Rsk1 Thr359/Ser363 phosphorylation in human cervical cancer tissue, normal kidney, liver, and small intestine tissue sections.
Peptide Inhibition Assay: Preincubation with the immunogen peptide, but not the corresponding non-phosphorylated control peptide, prevented the polyclonal antibody from detecting the target Rsk1 phosphorylation band.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a human Rsk1-derived sequence containing phosphorylated Thr359 and Ser363 residues.
Epitope pThr359/Ser363.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificitySpecificity of this polyclonal antibody was verified by immunogen peptide blocking in Western blotting analysis of EGF-induced target phosphorylation. The target phosphorylation sites Thr359/Ser363 are numbered based on human Rsk1 spliced isoform 1 (UniProt Q15418-1). Equivalent sites in spliced isoform 2, 3, and 4 are Thr368/Ser372 (UniProt Q15418-2), Thr267/Ser271 (UniProt Q15418-3), and Thr343/Ser347 (UniProt Q15418-4), respectively.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Xenopus, Bovine, Porcine, Chicken based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RPS6KA1
  • MAPKAPK1A
  • RSK1
Modifications
  • Phosphorylation
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~75 kDa observed. 82.72 kDa (human isoform 1), 83.93 kDa (human isoform 2), 72.70 kDa (human isoform 3), and 81.15 kDa (human isoform 4) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected enhanced Rsk1 Thr359/Ser363 phosphorylation in 10 µg of lysate from EGF-treated A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABS1849 04055977349412

Documentation

Anti-phospho-Rsk1 (Thr359/Ser363) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Rsk1 (Thr359/Ser363) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-Rsk1 (Thr359/Ser363) - 3195391 3195391
Anti-phospho-Rsk1 (Thr359/Ser363) - 3425387 3425387
Anti-phospho-Rsk1 (Thr359/Ser363) - 3500935 3500935
Anti-phospho-Rsk1 (Thr359/Ser363) - 3890508 3890508
Anti-phospho-Rsk1 (Thr359/Ser363) -Q2676855 Q2676855
Anti-phospho-Rsk1 (Thr359/Ser363) Polyclonal Antibody 3118632