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ABS1506-25UL Anti-phospho-SIN1 (Thr398)

Anti-phospho-SIN1 (Thr398), Cat. No. ABS1506, is a rabbit polyclonal antibody that detects Target of rapamycin complex 2 subunit MAPKAP1 (SIN1) and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Anti-phospho-SIN1 (Thr398) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: MAPKAP1, MIP1, SIN1 UniProt Number: Q9BPZ7
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ABS1506-25UL
25 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS1506-25UL
DescriptionAnti-phospho-SIN1 (Thr398)
Alternate Names
  • Target of rapamycin complex 2 subunit MAPKAP1
  • TORC2 subunit MAPKAP1
  • Mitogen-activated protein kinase 2-associated protein 1
  • Stress-activated map kinase-interacting protein 1
  • SAPK-interacting protein 1
  • mSIN1
Background InformationTarget of rapamycin complex 2 subunit MAPKAP1 (UniProt: Q9BPZ7; also known as TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1) is encoded by the MAPKAP1 (also known as MIP1, SIN1) gene (Gene ID: 79109) in human. mSIN1 is a subunit of mTORC2 regulates cell growth and survival in response to hormonal signals. It is ubiquitously expressed, with highest levels in heart and skeletal muscle. Six isoforms of SIN1 have been described that are produced by alternative splicing. All isoforms with the exception of isoform 4 can be incorporated into the mTORC2 complex. mTORC2 is shown to be activated by growth factors, but in contrast to mTORC1, it is nutrient-insensitive. mTORC2 phosphorylates serine 473 in Akt1, which facilitates the phosphorylation of Threonine 308 by PDK1, thereby allowing full activation of Akt. Genetic knockout of SIN1 is reported to abolish serine 473 phosphorylation of Akt and disrupts the RICTOR-mTOR interaction. mTORC2 also regulates the phosphorylation of SGK1 at serine 422. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and auto-phosphorylation.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-SIN1 (Thr398), Cat. No. ABS1506, is a rabbit polyclonal antibody that detects Target of rapamycin complex 2 subunit MAPKAP1 (SIN1) and has been tested for use in Western Blotting and Peptide Inhibition Assay.
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesPeptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with L6 cells treated with IGF-1 for peptide block analysis.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphorylated Threonine 398 from human Stress-activated MAP kinase-interacting protein 1 (SIN1).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects human Stress-activated MAP kinase-interacting protein 1 phosphorylated on Threonine 398.
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MAPKAP1
  • MIP1
  • SIN1
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~65 kDa observed; 59.12 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in L6 cells treated with IGF-1.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-SIN1 (Thr398) in lysate from L6 cells treated with IGF-1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABS1506-25UL 04054839431647