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ABE1392 Anti-phospho SIRT1 (Ser682) Antibody

Anti-phospho SIRT1 (Ser682) Antibody, Cat. No. ABE1392, is a highly specific rabbit Polyclonal antibody, that targets SIRT1 and has been tested in Western Blotting.

Anti-phospho SIRT1 (Ser682) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: SIRT1, SIR2L1 UniProt Number: Q96EB6
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ABE1392
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1392
DescriptionAnti-phospho SIRT1 (Ser682) Antibody
Alternate Names
  • NAD-dependent protein deacetylase sirtuin-1
  • hSIRT1
  • Regulatory protein SIR2 homolog 1
  • SIR2-like protein 1
  • hSIR2
  • SirtT1 75 kDa fragment
  • 75SirT1
  • phospho SIRT1 (Ser682)
Background InformationNAD-dependent protein deacetylase sirtuin-1 (UniProt Q96EB6; also known as hSIR2, hSIRT1, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, Sir2-like 1, SIR2-like protein 1, SIR2alpha, Sirtuin type 1) is encoded by the SIRT1 (also known as SIR2L1) gene (Gene ID 23411) in human. Sirtuins constitute a family of NAD+-dependent deacetylases found in nearly all organisms studied. Sirtuins have been implicated in the regulation of multiple processes, including aging, transcription, apoptosis, and stress resistance. Full-length SirT1 (FLSirT1) plays an important role in human cartilage homeostasis and chondrocyte survival by mediating the expression of major cartilage anabolic components, collagen 2(I) and aggrecan. The generation of the transcriptionally inactive 75-kDa fragment (75SirT1) via site-specific cleavage of FLSirT1 at amino acid 533 by cathepsin B is an anti-apoptotic mechanism to promote human osteoarthritic (OA) chondrocytes survival following exposure to proinflammatory cytokines. The 5SirT1 fragment is shown to associate with cytochrome c and likely blocks downstream apoptosome assembly. Phosphorylation plays an important role in modulating SirT1 activity. Multiple kinases and their corresponding SirT1 phosphorylation sites have been identified, including pT344 targeted by AMPK, pT530 and pS540 targeted by Cdk1/Cyclin B, pS46 (mouse) or pS47 (human) targeted by JNK1, pT522 targeted by DYRK1A & DYRK3, as well as pS27 and pS47 targeted by CaMKKβ.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho SIRT1 (Ser682) Antibody, Cat. No. ABE1392, is a highly specific rabbit Polyclonal antibody, that targets SIRT1 and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human phospho SIRT1 (Ser682) near the C-terminus.
EpitopeNear C-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificitySer682 of hSIRT1 isoform 1 is equivalent to Ser496 of hSIRT1 spliced isoform 2 (delta-exon8).
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Porcine, Feline, Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SIRT1
  • SIR2L1
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~110 kDa observed. The target band size appears larger than the calculated molecular weight (81.7 kDa/isoform 1 & 61.1 kDa/isoform 2). Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Nocodazole treated H1299 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected phospho SIRT1 (Ser682) in 10 µg of Nocodazole treated cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE1392 04055977176452

Documentation

Anti-phospho SIRT1 (Ser682) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho SIRT1 (Ser682) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho SIRT1 (Ser682) - 3425415 3425415
Anti-phospho SIRT1 (Ser682) - 4158032 4158032
Anti-phospho SIRT1 (Ser682) -Q2581259 Q2581259

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.