Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

07-1817 Anti-phospho-SRPK2 (Ser494) Antibody

Anti-phospho-SRPK2 (Ser494) Antibody is an antibody against phospho-SRPK2 (Ser494) for use in WB, PIA.

Anti-phospho-SRPK2 (Ser494) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: SRPK2 UniProt Number: P78362
View Products on Sigmaaldrich.com
07-1817
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, XnWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1817
DescriptionAnti-phospho-SRPK2 (Ser494) Antibody
Alternate Names
  • Serine/threonine-protein kinase SRPK2
  • SFRS protein kinase 2
  • Serine/arginine-rich protein-specific kinase 2
  • SR-protein-specific kinase 2
Background InformationAlong with Serine/threonine protein kinase 2 (SRPK2), SRPK1 phosphorylates serine residues found in RS-domain-containing proteins, such as SFRS1 and SFRS2. These kinases also phosphorylate the same serine residues on the HBV core protein both in vitro and in vivo. The cytoplasmic proteins are critical for nuclear import of SR proteins in a phosphorylation-dependent manner. Additionally, they mediate the trafficking of splicing factors and play a role in spliceosome assembly and mitogenesis.
References
Product Information
FormatAffinity Purified
Control
  • Untreated and insulin treated HepG2 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-SRPK2 (Ser494) Antibody is an antibody against phospho-SRPK2 (Ser494) for use in WB, PIA.
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesPeptide Inhibition Analysis: 1 µg/mL from a representative lot detected SRPK2 in 10 µg of insulin treated HepG2 cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the protein kinase domain of SRPK2 phosphorylated at Ser494.
EpitopeProtein kinase domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody is specific for the protein kinase domain of SRPK2 phosphorylated at Ser494, independent of observed phosphorylation at Ser497.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Xenopus
Species Reactivity NoteDemonstrated to react with Human and Mouse. Predicted to react with Rat and Xenopus based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SRPK2
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Phosphorylates RS domain-containing proteins, such as SFRS1 and SFRS2 on serine residues. Role in spliceosome assembly and in mediating the trafficking of splicing factors. Appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

COFACTOR: Magnesium.

ENZYME REGULATION: Activated by phosphorylation on Ser-52 and Ser-588.

SUBCELLULAR LOCATION: Cytoplasm. Nucleus.

TISSUE SPECIFICITY: Highly expressed in brain, moderately expressed in heart and skeletal muscle and at low levels in lung, liver, and kidney.
Molecular Weight~120 kDa observed. Two isoforms at ~77 kDa and ~79 kDa may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in untreated and insulin treated HepG2 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SRPK2 in 10 µg HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-1817 04053252437175

Documentation

Anti-phospho-SRPK2 (Ser494) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-SRPK2 (Ser494) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-SRPK2 (Ser494) - 2318814 2318814
Anti-phospho-SRPK2 (Ser494) - 2914311 2914311
Anti-phospho-SRPK2 (Ser494) - 3207100 3207100
Anti-phospho-SRPK2 (Ser494) - 3313737 3313737
Anti-phospho-SRPK2 (Ser494) - 3432914 3432914
Anti-phospho-SRPK2 (Ser494) - 3503872 3503872
Anti-phospho-SRPK2 (Ser494) - 3680722 3680722
Anti-phospho-SRPK2 (Ser494) - 3704779 3704779
Anti-phospho-SRPK2 (Ser494) - Q1970940 Q1970940
Anti-phospho-SRPK2 (Ser494) -2583445 2583445
Show more