Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

07-2268 Anti-phospho-TBC1D1 Antibody (Ser237)

Anti-phospho-TBC1D1 (Ser237), Cat. No. 07-2268, is a rabbit polyclonal antibody that detects TBC1D1 phosphorylated on serine 237 and is tested for use in Peptide Inhibition Assay and Western Blotting.

Anti-phospho-TBC1D1 Antibody (Ser237) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Insulin/Energy Signaling, Glucose/Glycogen Metabolism Gene Symbol: TBC1D1, TBC, TBC1 UniProt Number: Q86TI0
View Products on Sigmaaldrich.com
07-2268
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-2268
DescriptionAnti-phospho-TBC1D1 Antibody (Ser237)
Alternate Names
  • TBC1 domain family member 1
  • TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
Background InformationTBC1 domain family member 1 (UniProt: Q86TI0; also known as TBC1D1) is encoded by the TBC1D1 gene (Gene ID: 23216) in human. TBC1D1 is a member of the TBC1 Rab-GTPase family of nuclear proteins that is highly expressed in skeletal muscle. Its major action is to regulate exercise-mediated glucose uptake into muscle. It is involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells. Insulin and muscle contraction are reported to increase TBC1D1 phosphorylation on phospho-Akt substrate motifs. Insulin-stimulated phosphorylation by AKT is shown to stimulate GLUT4 translocation. Following muscle exercise, phosphorylation of TBC1D1 at serine 237 by AMPK and 14-3-3 protein binding capacity are significantly increased. Knockdown of TBC1D1 in C2C12 muscle cells is shown to increase fatty acid oxidation and reduce muscle levels of GLUT4. Although TBC1D1 knockout mice do not exhibit any disruption in their whole-body insulin action, they display impaired exercise-regulated metabolism. A mutation in TBC1D1 (R125W) has been linked to obesity in human. (Ref.: Stockli, J., et al. (2015). Diabetes. 64(6); 1914-1922; An, D., et al. (2010). Diabetes. 59(6); 1358-1365; Frosig, C., et al. (2010). J. Physiol. 588(22); 4539-4548).
References
Product Information
FormatAffinity Purified
Control
  • Lambda phosphatase treated and untreated HEK293 cell lysates
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-phospho-TBC1D1 (Ser237), Cat. No. 07-2268, is a rabbit polyclonal antibody that detects TBC1D1 phosphorylated on serine 237 and is tested for use in Peptide Inhibition Assay and Western Blotting.
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesTested Applications

Peptide Inhibition Assay: Target band detection in HEK293 cell lysate was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 237 of human TBC1 domain family member 1 (TBC1D1).
EpitopePhosphorylated Ser237
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects TBC1D1 phosphorylated on serine 237.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Monkey, Chimpanzee, Mouse, Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryTBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334), yeast Bub2, and CDC16 (MIM 603461) (White et al., 2000 [PubMed 10965142]).[supplied by OMIM].
Gene Symbol
  • TBC1D1
  • TBC
  • TBC1
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues (By similarity).

SUBCELLULAR LOCATION: Nucleus (By similarity).

SEQUENCE SIMILARITIES: Contains 1 PID domain.

Contains 1 Rab-GAP TBC domain.

SEQUENCE CAUTION: The sequence BAB55057.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight~158 kDa observed; 133.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells treated with phorbol-12-myristate-13-acetate
(PMA).

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected TBC1D1 lysate from HEK293 cells treated with phorbol-12-myristate-13-acetate (PMA). However, the detection was inhibited blots pre-incubated with protein phosphatase.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-2268 04053252292149

Documentation

Anti-phospho-TBC1D1 Antibody (Ser237) SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-TBC1D1 Antibody (Ser237) Certificates of Analysis

TitleLot Number
Anti-phospho-TBC1D1 (Ser237) - 2390581 2390581
Anti-phospho-TBC1D1 (Ser237) - 2427514 2427514
Anti-phospho-TBC1D1 (Ser237) - 2061452 2061452
Anti-phospho-TBC1D1 (Ser237) - 2204971 2204971
Anti-phospho-TBC1D1 (Ser237) - 2273986 2273986
Anti-phospho-TBC1D1 (Ser237) - 2562374 2562374
Anti-phospho-TBC1D1 (Ser237) - 3286488 3286488
Anti-phospho-TBC1D1 (Ser237) - 3393428 3393428
Anti-phospho-TBC1D1 (Ser237) - 3517960 3517960
Anti-phospho-TBC1D1 (Ser237) - 3692729 3692729
Show more