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ABF131 Anti-phospho TIE2/TEK Antibody (Tyr992)

This Anti-phospho TIE2/TEK Antibody (Tyr992) is validated for use in western blotting, IP & peptide inhibition assay for the detection of phospho TIE2/TEK (Tyr992).

Anti-phospho TIE2/TEK Antibody (Tyr992) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: TEK, TIE2, VMCM, VMCM1 UniProt Number: Q02763
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ABF131
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABF131
Replaces09-407
DescriptionAnti-phospho TIE2/TEK Antibody (Tyr992)
Alternate Names
  • Angiopoietin-1 receptor
  • Endothelial tyrosine kinase
  • Tunica interna endothelial cell kinase
  • Tyrosine kinase with Ig and EGF homology domains-2
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • hTIE2
  • p140 TEK
  • CD202b
Background InformationTIE2/TEK (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2/TEK are associated with inherited venous malformations; the TIE2/TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho TIE2/TEK Antibody (Tyr992) is validated for use in western blotting, IP & peptide inhibition assay for the detection of phospho TIE2/TEK (Tyr992).
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Peptide Inhibition Assay
Application NotesImmunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated phospho TIE2/TEK (Tyr992) from Pervanadate treated HUVEC cell lysate. Immunoprecipated sample was then subjected to Western Blotting using a 1:5,000 dilution of this antibody.

Peptide Inhibition Assay Analysis: A 1:500 dilution from a representative lot detected phospho TIE2/TEK (Tyr992) from transfected HEK293T lysates when pre-incubated without or with non-phospho peptide, and not when pre-incubated with phospho peptide.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the Protein Kinase Domain of human TIE2/TEK phosphorylated at Tyr992.
EpitopeProtein Kinase Domain
Concentration0.25 mg/mL
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine, and Zebrafish based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TEK
  • TIE2
  • VMCM
  • VMCM1
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~125 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in untreated and Pervanadate treated HUVEC cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho TIE2/TEK (Tyr992) in 10 µg of Pervanadate treated HUVEC cell lysate, and not in untreated HUVEC cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABF131 04053252972928

Documentation

Anti-phospho TIE2/TEK Antibody (Tyr992) SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho TIE2/TEK Antibody (Tyr992) Certificates of Analysis

TitleLot Number
Anti-phospho TIE2/TEK (Tyr992) 3073761
Anti-phospho TIE2/TEK (Tyr992) - Q2420919 Q2420919
Anti-phospho TIE2/TEK (Tyr992) - 3434784 3434784
Anti-phospho TIE2/TEK (Tyr992) - 3517907 3517907
Anti-phospho TIE2/TEK (Tyr992) - 3758615 3758615
Anti-phospho TIE2/TEK (Tyr992) -2683262 2683262
Anti-phospho TIE2/TEK (Tyr992) -2788125 2788125
Anti-phospho TIE2/TEK (Tyr992) Polyclonal Antibody 2953822
Anti-phospho TIE2/TEK (Tyr992) Polyclonal Antibody 2887486
Anti-phospho TIE2/TEK (Tyr992) Polyclonal Antibody 2865446
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