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ABS219 Anti-phospho-Tie2 (Ser1119) Antibody

Anti-phospho-Tie2 (Ser1119) Antibody is an antibody against phospho-Tie2 (Ser1119) for use in WB.

Anti-phospho-Tie2 (Ser1119) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: TEK, TIE2 UniProt Number: Q02763
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ABS219
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, B, M, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS219
DescriptionAnti-phospho-Tie2 (Ser1119) Antibody
Alternate Names
  • Angiopoietin-1 receptor
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • hTIE2
  • p140 TEK
  • CD202b
Background InformationTIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
References
Product Information
FormatAffinity Purified
Control
  • HEK293T overexpressing Tie2 protein
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Tie2 (Ser1119) Antibody is an antibody against phospho-Tie2 (Ser1119) for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Tie2 phosphorylated at Ser1119.
EpitopePhosphorylated Ser1119
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Tie2 phosphorylated at Ser1119.
Species Reactivity
  • Human
  • Bovine
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Bovine, Mouse, and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq, Jul 2008].
Gene Symbol
  • TEK
  • TIE2
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.

CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

SUBUNIT STRUCTURE: Interacts with PTPRB.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

TISSUE SPECIFICITY: Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.

PTM: Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.

Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells (By similarity).

INVOLVEMENT IN DISEASE: Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.

SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

Contains 3 EGF-like domains.

Contains 3 fibronectin type-III domains.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 protein kinase domain.
Molecular Weight~160 kDa observed. Uniprot gives a calculated molecular weight of 126 kDa, but has been observed at ~160 kDa due to glycosolation. A non-modified specific band appears at ~52 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HEK293T overexpressing Tie2 protein.

Western Blot Analysis: A 1:500 dilution detected Tie2 on 10 µg of HEK293T overexpressing Tie2 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABS219 04053252592461

Documentation

Anti-phospho-Tie2 (Ser1119) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Tie2 (Ser1119) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-Tie2 (Ser1119) - 2266772 2266772
Anti-phospho-Tie2 (Ser1119) - 2281675 2281675
Anti-phospho-Tie2 (Ser1119) - 3198461 3198461
Anti-phospho-Tie2 (Ser1119) - 3205951 3205951
Anti-phospho-Tie2 (Ser1119) - 3237542 3237542
Anti-phospho-Tie2 (Ser1119) - 3753314 3753314
Anti-phospho-Tie2 (Ser1119) - NRG1929418 NRG1929418
Anti-phospho-Tie2 (Ser1119) -2720124 2720124
Anti-phospho-Tie2 (Ser1119) -2803237 2803237
Anti-phospho-Tie2 (Ser1119) Polyclonal Antibody 3105316
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Product Families

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The thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall.
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies