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16-235 Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 488

This Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor 488 is validated for use in FC, IC for the detection of phospho-erbB-2/HER-2 (Tyr1248).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors, Kinases & Phosphatases Gene Symbol: ERBB2, HER2, HER-2, NGL, herstatin, erbB-2, C-erbB-2, NEU, TKR1, p185erbB2, HER-2/neu UniProt Number: P04626
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16-235
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RFC, ICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue Number16-235
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 488
References
Product Information
FormatAlexaFluor®488
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor 488 is validated for use in FC, IC for the detection of phospho-erbB-2/HER-2 (Tyr1248).
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Biological Information
ImmunogenSynthetic phosphopeptide, residues 1243-1255, corresponding to a major autophosphorylation (Tyr1248) site of human Erb B2/HER-2 (C-AENPEpYLGLDVPV)
HostRabbit
Specificitythe activated form of Erb B2/HER-2 oncoprotein
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Species Reactivity NotePredicted to cross react with rat based on conservation of immunogenic sequence
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Gene Symbol
  • ERBB2
  • HER2
  • HER-2
  • NGL
  • herstatin
  • erbB-2
  • C-erbB-2
  • NEU
  • TKR1
  • p185erbB2
  • HER-2/neu
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
SIZE: 1255 amino acids; 137910 Da
SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
Molecular Weight185kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunostaining of EGF treated A431 cells
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at 4°C from date of shipment
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
16-235 04053252507663

Documentation

Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 488 SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 488 Certificates of Analysis

TitleLot Number
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluor#174; 488 conjugate (rabbit immunoaffinity purified IgG) - 2108474 2108474
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluor#174; 488 conjugate - DAM1567754 DAM1567754
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluor#174; 488 conjugate - JBC1920618 JBC1920618
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluorreg; 488 conjugate - 30489 30489
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluorreg; 488 conjugate - 33437 33437
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluor® 488 conjugate (rabbit immunoaffinity purified IgG) - 2424085 2424085
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluor® 488 conjugate - 3171756 3171756
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluor® 488 conjugate - 3919695 3919695
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluor® 488 conjugate -2547169 2547169
Anti-phospho-erbB-2/HER-2 (Tyr1248), Alexa Fluor® 488 conjugate -2630274 2630274

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Alternative Format

Catalog Number Description
06-229 Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody
16-236 Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody, Alexa Fluor® 555

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies