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MABS64 Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2

Detect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: SAPK2a, MAPK14, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, p38ALPHA, RK, Mxi2, EXIP UniProt Number: Q16539
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MABS64
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS64
DescriptionAnti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2
Alternate Names
  • mitogen-activated protein kinase 14
  • cytokine suppressive anti-inflammatory drug binding protein
  • stress-activated protein kinase 2A
  • Cytokine suppressive anti-inflammatory drug-binding protein
  • CSAID-binding protein
  • MAP kinase MXI2
  • MAX-interacting protein 2
  • CSAID-binding protein
  • Mitogen-activated protein kinase p38 alpha
  • p38 MAP kinase
  • p38 mitogen activated protein kinase
  • MAP kinase p38 alpha
  • Csaids binding protein
Background InformationThe p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Anisomycin treated and untreated HeLa cell lysate
PresentationPurified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect phospho-p38 (Thr180/Tyr182) using this Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 validated for use in WB & IC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: 1:500 dilution from a previous lot detected p38 in Anisomycin treated NIH/3T3 cells.
Biological Information
ImmunogenLinear peptide corresponding to human p38 phosphorylated at Thr180 and Tyr182.
EpitopePhosphorylated Thr180 and Tyr182
Clone6E5.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes p38 when phosphorylated at Thr180 and Tyr182.
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SAPK2a
  • MAPK14
  • CSBP1
  • CSBP2
  • CSPB1
  • PRKM14
  • PRKM15
  • p38ALPHA
  • RK
  • Mxi2
  • EXIP
Modifications
  • Phosphorylation
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.

COFACTOR: Magnesium.

SIZE: 360 amino acids; 41293 Da

SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (by similarity). Interacts with NP60.

SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).

TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR.

SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight41 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Anisomycin treated and untreated HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected p38 on 10 µg of Anisomycin treated and untreated HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS64 04053252478741

Documentation

Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-p38 (Thr180/Tyr182) Antibody, clone 6E5.2 Certificates of Analysis

TitleLot Number
Anti-phospho-p38 (Thr180/Tyr182), -2768416 2768416
Anti-phospho-p38 (Thr180/Tyr182), clone 6E5.2 - 2384015 2384015
Anti-phospho-p38 (Thr180/Tyr182), clone 6E5.2 - 2016659 2016659
Anti-phospho-p38 (Thr180/Tyr182), clone 6E5.2 - 2080896 2080896
Anti-phospho-p38 (Thr180/Tyr182), clone 6E5.2 - 2192181 2192181
Anti-phospho-p38 (Thr180/Tyr182), clone 6E5.2 - 2239623 2239623
Anti-phospho-p38 (Thr180/Tyr182), clone 6E5.2 - 2300631 2300631
Anti-phospho-p38 (Thr180/Tyr182), clone 6E5.2 - 2828589 2828589
Anti-phospho-p38 (Thr180/Tyr182), clone 6E5.2 - 3862686 3862686
Anti-phospho-p38 (Thr180/Tyr182), clone 6E5.2 - 3991566 3991566
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