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07-449-AF647 Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate Antibody

This Anti-trimethyl Histone H3 (Lys27) antibody is validated for use in ICC for the detection of trimethyl Histone H3 (Lys27).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
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07-449-AF647
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCRbAlexaFluor®647Polyclonal Antibody
Description
Catalogue Number07-449-AF647
DescriptionAnti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate Antibody
Alternate Names
  • Histone H3.1t
  • H3/t
  • H3t
  • H3/g
Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 07-449.
References
Product Information
FormatAlexaFluor®647
PresentationPurified rabbit conjugate antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-trimethyl Histone H3 (Lys27) antibody is validated for use in ICC for the detection of trimethyl Histone H3 (Lys27).
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenLinear peptide corresponding to Histone H3 trimethylated at Lys27.
Concentration0.5 mg/mL
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight~17 kDa observed (Refer to Cat. No. 07-449)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry Analysis: in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl Histone H3 (Lys27) in HeLa cells.

Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-449-AF647 04053252971594

Documentation

Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate Antibody Certificates of Analysis

TitleLot Number
Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate 2864845
Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate - Q2418596 Q2418596
Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate - 3419564 3419564
Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate - 4133768 4133768
Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate -2523619 2523619
Anti-trimethyl Histone H3 (Lys27), Alexa Fluor® 647 Conjugate -2616194 2616194

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Life Science Research > Antibodies and Assays > Primary Antibodies