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ABE435-S Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size

Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size, is a rabbit polyclonal antibody for detection of Histone H3 trimethylated at lysine 36 (H3K36me3). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ICC. Small pack size is ideal for screening applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, HIST3H3, H3FT, H3T, MGC126886 UniProt Number: Q16695
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ABE435-S
20 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, DB, ChIP, ChIP-seqRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE435-S
DescriptionAnti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size
Alternate Names
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/I
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l
  • H3K36me3
Background InformationHistones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Methylation occurs on histone H3 at lysine 4, 9, 27, 36, and 79 residues. Previous studies have reported that methylation of H3 lysine 9 provides a binding site for heterochromatin adaptor proteins (HP1) which are involved in the formation of heterochromatin and gene repression. Phosphorylation of histones may also produce similar repressive effects depending on the cellular context. Often, it is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
References
Product Information
FormatAffinity Purified
Control
  • HeLa cell extract and recombinant Histone H3 protein
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size, is a rabbit polyclonal antibody for detection of Histone H3 trimethylated at lysine 36 (H3K36me3). This purified Ab is specificity verified by dot blot (DB) and validated in WB, ICC. Small pack size is ideal for screening applications.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Dot Blot
  • Chromatin Immunoprecipitation (ChIP)
  • ChIP-seq
Application NotesDot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-trimethyl-Histone H3 (Lys36) (1:2,000 dilution).

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Histone H3 in A431 cells.

ChIP-Sequencing: Chromatin immunoprecipitation was performed using the Magna ChIP™ HiSens kit (cat#17-10460, 2 µg of anti-trimethyl-Histone H3 (Lys36) antibody(ABE435-S), 20 µL Protein A/G beads , and 1e6 crosslinked HeLa cell chromatin followed by DNA purification using magnetic beads. Libraries were prepared from Input and ChIP DNA samples using standard protocols with Illumina barcoded adapters, and analyzed on Illumina HiSeq™ instrument. An excess of eleven million reads from FastQ files were mapped using Bowtie (http://bowtie-bio.sourceforge.net/manual.shtml) following TagDust (http://genome.gsc.riken.jp/osc/english/dataresource/) tag removal. Peaks were identified using MACS (http://luelab.dfci.harvard.edu/MACS/), with peaks and reads visualized as a custom track in UCSC Genome Browser (http://genome.ucsc.edu) from BigWig and BED files.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3 trimethylated at Lys36.
EpitopeTrimethylated Lys36
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Histone H3 trimethylated at Lys36.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteDemonstrated to react with Human and Mouse. Broad species reactivity expected due to similar sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
  • HIST3H3
  • H3FT
  • H3T
  • MGC126886
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~17 kDa observed. An uncharacterized band appears at ~52 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell extract and recombinant Histone H3 protein.

Western Blot Analysis: A 1:500 dilution of this antibody detected Histone H3 on 10 µg of HeLa cell extract and recombinant Histone H3 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size20 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE435-S 04053252907579

Documentation

Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size SDS

Title

Safety Data Sheet (SDS) 

Anti-trimethyl-Histone H3 (Lys36) Antibody, Trial Size Certificates of Analysis

TitleLot Number
Anti-trimethyl Histone H3 (Lys36), -2545779 2545779
Anti-trimethyl Histone H3 (Lys36), Trial Size - 3297983 3297983
Anti-trimethyl Histone H3 (Lys36), Trial Size - 3639191 3639191
Anti-trimethyl Histone H3 (Lys36), Trial Size - Q2188004 Q2188004
Anti-trimethyl Histone H3 (Lys36), Trial Size -2564877 2564877
Anti-trimethyl Histone H3 (Lys36), Trial Size -2710625 2710625
Anti-trimethyl Histone H3 (Lys36), Trial Size Polyclonal Antibody 3122443
Anti-trimethyl Histone H3 (Lys36), Trial Size Polyclonal Antibody 2853636

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies