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07-473-AF488 Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 488 Conjugate

This Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
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07-473-AF488
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue Number07-473-AF488
DescriptionAnti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 488 Conjugate
Alternate Names
  • H3K4me3
  • Histone H3 (trimethyl Lys4)
  • H3 histone family, member T
  • Histone 3, H3
  • Histone cluster 3, H3
Background InformationHistone H3 is one of the five main histone proteins that are involved in the structure of chromatin in eukaryotic cells. Histone H3 features a main globular domain and a long N-terminal tail. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 is specifically phosphorylated during both mitosis and meiosis in patterns that are specifically coordinated in space and time. These phosphorylations are initiated at different phases of the cell division. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit conjugate antibody in buffer containing PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in A431 and NIH/3T3 cells.
The unconjugated version of this antibody has been shown to work in ChIP, DB, WB, ChIP-seq.
Biological Information
ImmunogenSynthetic peptide corresponding to residues surrounding and including trimethlyated Lys 4 of Histone H3.
EpitopeTrimethylated (Lys4)
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Vertebrates based on 100% sequence homology. Expected to react with most other species based on extreme conserved homology among species.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Modifications
  • Methylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~16 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys4) in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-473-AF488 04055977182019

Documentation

Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 488 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 488 Conjugate Certificates of Analysis

TitleLot Number
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 3140203
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 2956745
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 3290736
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 - 3515597 3515597
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 - 3707320 3707320
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 - 3752724 3752724
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 - 4138369 4138369
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 - 4181424 4181424
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 -2771834 2771834
Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 488 -2851079 2851079
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Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.