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OP01 Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259)

Overview

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RRMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
OP01-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes both phosphorylated and nonphosphorylated forms of v-H-Ras, and v-K-Ras in SW480 and Y1 cells and normal skin tissue. Also recognizes H, K, and N-Ras human translated products.
Catalogue NumberOP01
Brand Family Calbiochem®
References
ReferencesShimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive controlSW480 or Y1 cells or normal skin tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Neutralization Studies
Paraffin Sections
Application NotesFrozen Sections (5 µg/ml)
Immunoblotting (2.5 µg/ml, colormetric or chemiluminescence)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (use Cat. No. OP01A or 1 µg antibody per sample)
Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required)
Neutralization Studies (see application references)
Application CommentsBroadly reactive with the phosphorylated and non-phosphorylated forms of v-H-Ras and v-K-Ras (see application references) and recognizes the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl v-H-ras (Ab-1) agarose conjugate (Cat. No. OP01A) is recommended to be used as an immunoconcentrator prior to immunoblot analysis of p21ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant p21 Ras
Epitopewithin amino acids 62-76
CloneY13-259
HostRat
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalog Number GTIN
OP01-100UG 04055977209570