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189490 Autophagy Inhibitor, 3-MA - CAS 5142-23-4 - Calbiochem

Autophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker that protects cerebellar granule cells from apoptosis following serum/potassium deprivation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3-Methyladenine

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189490
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
5142-23-4C₆H₇N₅

Products

Catalog NumberPackaging Qty/Pack
189490-100MG Glass bottle 100 mg
189490-50MG Plastic ampoule 50 mg
Description
OverviewA widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)
Catalogue Number189490
Brand Family Calbiochem®
Synonyms3-Methyladenine
References
ReferencesCanu, N., et al. 2005. J. Neurochem. 92, 1228.
Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun. 316, 845.
Beugnet, A., et al. 2003. Biochem. J. 372, 555.
Eskelinen, E.L., et al. 2002. Traffic 3, 878.
Petiot, A., et al. 2000. J. Biol. Chem. 275, 992.
Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA 79, 1889.
Product Information
CAS number5142-23-4
FormWhite to faint yellow powder
Hill FormulaC₆H₇N₅
Chemical formulaC₆H₇N₅
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationAutophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker that protects cerebellar granule cells from apoptosis following serum/potassium deprivation.
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
189490-100MG 04055977221831
189490-50MG 07790788048235

Documentation

Autophagy Inhibitor, 3-MA - CAS 5142-23-4 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Autophagy Inhibitor, 3-MA - CAS 5142-23-4 - Calbiochem Certificates of Analysis

TitleLot Number
189490

References

Reference overview
Canu, N., et al. 2005. J. Neurochem. 92, 1228.
Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun. 316, 845.
Beugnet, A., et al. 2003. Biochem. J. 372, 555.
Eskelinen, E.L., et al. 2002. Traffic 3, 878.
Petiot, A., et al. 2000. J. Biol. Chem. 275, 992.
Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA 79, 1889.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-May-2012 JSW
Synonyms3-Methyladenine
DescriptionA widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)
FormWhite to faint yellow powder
Intert gas (Yes/No) Packaged under inert gas
CAS number5142-23-4
Chemical formulaC₆H₇N₅
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (10 mg/ml) or H₂O (5 mg/ml). In applications where high working concentrations are necessary (e.g. 10 mM), the use of a pre-made stock solution may result in the introduction of higher than desired solvent (e.g., DMSO) into the experimental systems. In such cases, it is recommended that the solid compound be weighed out at the time of use and reconstituted directly into the solutions used in the experimental system (buffers, media, etc.). Vortexing may be required for complete solubilization in water.
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesCanu, N., et al. 2005. J. Neurochem. 92, 1228.
Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun. 316, 845.
Beugnet, A., et al. 2003. Biochem. J. 372, 555.
Eskelinen, E.L., et al. 2002. Traffic 3, 878.
Petiot, A., et al. 2000. J. Biol. Chem. 275, 992.
Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA 79, 1889.