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71285 BCA Protein Assay Kit

The BCA protein assay is a simple and reliable protein quantification method.

BCA Protein Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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71285
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
71285-3 Glass bottle 500 assa
Description
OverviewThe BCA protein assay is based on a biuret reaction, which is the reduction of Cu2+ to Cu+ by proteins in an alkaline solution with concentration-dependent detection of the monovalent copper ions. Bicinchoninic acid is a chromogenic reagent that chelates the reduced copper, producing a purple complex with strong absorbance at 562 nm (Smith 1985, Wiechelman 1988). This assay can be used to quantify protein concentration with a wide variety of samples and can be performed in minutes.

The Novagen® BCA Protein Assay Kit can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration. Kit components are sufficient to complete 500 standard-size reactions (50 µl protein sample plus 1 ml reagent) or 2500 micro-scale reactions (25 µl protein sample plus 200 µl reagent). A BSA standard (2 mg/ml) is provided for convenient and reliable preparation of standard curves.

This assay is robust and can be performed in the presence of many compounds. Some reagents, including chelating agents, strong acids or bases, and reducing agents, interfere with the reduction and chelating reactions on which this assay depends (Brown 1989). The BCA assay is compatible with the following Novagen protein extraction and lysis reagents: BugBuster® Protein Extraction Reagent, PopCulture® Reagent, CytoBuster™ Protein Extraction Reagent, Reportasol™ Extraction Buffer, and Insect PopCulture Reagent. Options for the removal or dilution of interfering substances are described in the kit literature.
Catalogue Number71285
Brand Family Novagen®
References
ReferencesSmith, P.K., et al. 1985. Anal. Biochem. 150, 76.

Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.

Wiechelman, K., et al. 1988. Anal. Biochem. 175, 231.

Brown, R., et al. 1989. Anal. Biochem. 180, 136.
Product Information
•500 mlBCA Solution
•15 ml4% Cupric Sulfate
•3 x 1 mlBSA Standard, 2 mg/ml
DeclarationUse of the BCA protein assay is permitted for research purposes only.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
71285-3 07790788060435

Documentation

BCA Protein Assay Kit SDS

Title

Safety Data Sheet (SDS) 

BCA Protein Assay Kit Certificates of Analysis

TitleLot Number
71285

References

Reference overview
Smith, P.K., et al. 1985. Anal. Biochem. 150, 76.

Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem 159, 138.

Wiechelman, K., et al. 1988. Anal. Biochem. 175, 231.

Brown, R., et al. 1989. Anal. Biochem. 180, 136.

Brochure

Title
Protein Purification and Detection Tools

Technical Info

Title
Western Blotting Quick Reference Product Guide

Citations

Title
  • Matthias E. Liechti, et al. (2007) Metabotropic glutamate 2/3 receptors in the ventral tegmental area and the nucleus accumbens shell are involved in behaviors relating to nicotine dependence. Journal of Neuroscience 27, 9077-9085.
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    User Protocols

    Title
    TB380 BCA Protein Assay Kit