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196000 Bafilomycin A1, Streptomyces griseus - CAS 88899-55-2 - Calbiochem

This product can no longer be ordered through emdmillipore.com. Please refer to (Cat. No. 196000) on sigmaaldrich.com.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: vacuolar type H+ ATPase
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196000
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
88899-55-2C₃₅H₅₈O₉

Products

Catalog NumberPackaging Qty/Pack
196000-10UG Plastic ampoule 10 μg
196000-1SET Glass bottle 1 set
Description
OverviewA macrolide antibiotic that acts as a specific inhibitor of vacuolar-type H+-ATPase (V-type; Ki = 500 pM). A valuable tool for distinguishing among different types of ATPases. Blocks lysosomal cholesterol trafficking in macrophages and is known to interfere with pH regulation in brain cells. Exhibits cytotoxic effects on a number of cell lines in a cell viability assay. Reported to selectively inhibit β-secretase, an enzyme involved in the processing of amyloid precursor protein (APP). The InSolution™ format with a purity of ≥97% by HPLC in 90% DMSO is also available (Cat. No. 508409).
Note: 1 set = 3 x 10 µg
Catalogue Number196000
Brand Family Calbiochem®
References
ReferencesSinha, S., and Lieberburg, I. 1999. Proc. Natl. Acad. Sci. USA 96, 11049.
Calvert, C.M., and Sanders, D. 1995. J. Biol. Chem. 270, 7272.
Nishihara, T., et al. 1995. Biochem. Biophys. Res. Commun. 269, 255.
Crider, B.P., et al. 1994. J. Biol. Chem. 269, 17379.
Palokangas, H., et al. 1994. J. Biol. Chem. 269, 17577.
Sundquist, K.T., and Marks, S.C., Jr. 1994. J. Bone Miner. Res. 9, 1575.
Drose, S., et al. 1993. Biochemistry 32, 3902.
Furuchi, T., et al. 1993. J. Biol. Chem. 268, 27345.
Bowman, E.J., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7972.
Product Information
CAS number88899-55-2
FormWhite solid
Hill FormulaC₃₅H₅₈O₉
Chemical formulaC₃₅H₅₈O₉
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary Targetvacuolar type H+ ATPase
Primary Target IC<sub>50</sub>500 pM
Purity≥90% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Carcinogenic / Teratogenic
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 year at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
196000-10UG 04055977206456
196000-1SET 04055977206449

Documentation

Bafilomycin A1, Streptomyces griseus - CAS 88899-55-2 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Bafilomycin A1, Streptomyces griseus - CAS 88899-55-2 - Calbiochem Certificates of Analysis

TitleLot Number
196000

References

Reference overview
Sinha, S., and Lieberburg, I. 1999. Proc. Natl. Acad. Sci. USA 96, 11049.
Calvert, C.M., and Sanders, D. 1995. J. Biol. Chem. 270, 7272.
Nishihara, T., et al. 1995. Biochem. Biophys. Res. Commun. 269, 255.
Crider, B.P., et al. 1994. J. Biol. Chem. 269, 17379.
Palokangas, H., et al. 1994. J. Biol. Chem. 269, 17577.
Sundquist, K.T., and Marks, S.C., Jr. 1994. J. Bone Miner. Res. 9, 1575.
Drose, S., et al. 1993. Biochemistry 32, 3902.
Furuchi, T., et al. 1993. J. Biol. Chem. 268, 27345.
Bowman, E.J., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7972.

Technical Info

Title
Molecular Biology Grade Reagents
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-February-2010 JSW
DescriptionA macrolide antibiotic that acts as a specific inhibitor of vacuolar-type H+-ATPase (V-type; Ki = 0.5 nM) and is a valuable tool for distinguishing among different types of ATPases. Reported to selectivity inhibit β-secretase, an enzyme involved in the processing of amyloid precursor protein (APP). Blocks lysosomal cholesterol trafficking in macrophages. Known to interfere with pH regulation in brain cells. Inhibits bone resorption both in vivo and in vitro. Has been shown to exhibit cytotoxic effects on a number of cell lines in a cell viability assay.
FormWhite solid
CAS number88899-55-2
Chemical formulaC₃₅H₅₈O₉
Structure formulaStructure formula
Purity≥90% by HPLC
SolubilityDMSO (100 µM) or Ethanol
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 year at -20°C.
Toxicity Carcinogenic / Teratogenic
ReferencesSinha, S., and Lieberburg, I. 1999. Proc. Natl. Acad. Sci. USA 96, 11049.
Calvert, C.M., and Sanders, D. 1995. J. Biol. Chem. 270, 7272.
Nishihara, T., et al. 1995. Biochem. Biophys. Res. Commun. 269, 255.
Crider, B.P., et al. 1994. J. Biol. Chem. 269, 17379.
Palokangas, H., et al. 1994. J. Biol. Chem. 269, 17577.
Sundquist, K.T., and Marks, S.C., Jr. 1994. J. Bone Miner. Res. 9, 1575.
Drose, S., et al. 1993. Biochemistry 32, 3902.
Furuchi, T., et al. 1993. J. Biol. Chem. 268, 27345.
Bowman, E.J., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7972.