Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

SCT275 BioTracker™ Green Late-Phase Autophagy Live Cell Probe

Fluorescence microscopy
Flow cytometry

SCT275
20 nmol  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT275
Data setup Error. Pagelet <feature_brand-BioTracker> not found.
DescriptionBioTracker™ Green Late-Phase Autophagy Live Cell Probe
OverviewSCT275 is used to detect late phase autophagy in live cells. This small hydrophobic probe molecule is plasma membrane-permeable and incorporates in to the autophagosome. Following lysosome-autophagosome fusion, increased acidity in the autolysosome enhances fluorescence signal of the BioTracker™ Green Late-Phase Autophagy Live Cell Probe.

SPECTRAL CHARACTERISTICS:
Excitation λ: 405 nm
Emission λ: 525 nm

Recommended laser/filter:
Ex 350 - 450 nm
Em 500 nm - 560 nm
Alternate Names
  • green autophagy probe
  • autolysosome live cell probe
Background InformationAutophagy is an intracellular degradation process, where dysfunctional proteins and organelles are degraded by a system of organelles. Aggregated dysfunctional proteins are surrounded by the double membrane to form an autophagosome. DALGreen, which is a small hydrophobic molecule, passes the plasma membrane of live cells and is incorporated in the autophagosome.
References
Product Information
Detection methodFlow cytometry Fluorescence
FormatLyophilized solid
PresentationLyophilized solid.
Quality LevelMQ100
Applications
ApplicationFluorescence microscopy
Flow cytometry
Key Applications
  • Cell Based Assays
  • Fluorescence
  • Flow Cytometry
  • Fluorescence Activated Cell Sorting (FACS)
Application NotesSCT275 is a small hydrophobic molecule live cell probe that localizes to the autolysosome. The probe fluoresces green as the autolysosome environment acidifies, enabling the assessment of autophagy by fluorescence microscopy and flow cytometry.

Add 20 uL DMSO to tube of SCT275 and pipet to dissolve to make a 1 mM stock solution. Dilute with culture medium to 0.1 - 1.0 uM to make working solution.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20 °C, protected from light.
Packaging Information
Material Size20 nmol
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
SCT275 04065269218555

Documentation

Related Products & Applications