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343222 Biotin, Fluorescein Conjugate

Biotin, Fluorescein Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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343222
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
343222-1MG Plastic ampoule 1 mg
Description
Catalogue Number343222
Brand Family Calbiochem®
References
ReferencesGruber, H.J., et al. 1998. Biochim. Biophys. Acta 1381, 203
Product Information
FormLyophilized orange solid
Quality LevelMQ100
Applications
Key Applications Enzyme Immunoassay
Immunohistochemistry
Application NotesEIA (1:1,000)
Tissue Staining (1:50 - 1:100)
Application CommentsMake a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
343222-1MG 04055977195088

Documentation

Biotin, Fluorescein Conjugate SDS

Title

Safety Data Sheet (SDS) 

Biotin, Fluorescein Conjugate Certificates of Analysis

TitleLot Number
343222

References

Reference overview
Gruber, H.J., et al. 1998. Biochim. Biophys. Acta 1381, 203
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-September-2007 RFH
ApplicationEIA (1:1,000)
Tissue Staining (1:50 - 1:100)
DescriptionAvidin (and streptavidin) has 4 binding sites for biotin. Taking advantage of the high affinity between avidin and biotin, this fluorescein-conjugated biotin-X (a 6-atom spacer) provides a method for fluorescent detection of biotinylated antibodies.
FormLyophilized orange solid
Recommended reaction conditionsFor tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50-1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1000.
SolubilityDMF (5 mg/ml), DMSO (5 mg/ml), or Methanol (5 mg/ml)
CommentsMake a stock solution by dissolving in DMF (DMSO and methanol should also work). Suggested concentration for stock solution is 5 mg/ml. Dilute stock solution with appropriate buffer for assay just prior to use. For tissue staining, suggested usage is to dilute stock solution to 1 mg/ml and then dilute that solution 1:50 - 1:100. For EIA, follow the same procedure, but dilute the 1 mg/ml solution 1:1,000. Variables associated with assay conditions will dictate the proper working dilution.
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesGruber, H.J., et al. 1998. Biochim. Biophys. Acta 1381, 203