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203890 CRAC Channel Inhibitor, BTP2 - CAS 223499-30-7 - Calbiochem

CRAC Channel Inhibitor, BTP2, CAS 223499-30-7, is a cell-permeable, potent blocker of Stim1 and Orai1-coupled Ca2+ release-activated Ca2+ channel-mediated SOCE in RBL-2H3 cells (IC50 = 590 nM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: [N-{4-[3,5-bis(Trifluoromethyl)-1H-pyrazol-1-yl]phenyl}-4-methyl-1,2,3-thiadiazole-5-carboxamide], Pyr2, TPRC3 Channel Inhibitor II

Primary Target: CRAC Channel
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203890
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
223499-30-7C₁₅H₉F₆N₅OS

Products

Catalog NumberPackaging Qty/Pack
203890-5MG Plastic ampoule 5 mg
Description
OverviewA cell-permeable pyrazole that acts as a more potent blocker of Stim1 and Orai1-coupled CRAC (Ca2+ release-activated Ca2+) channel-mediated SOCE (store-operated Ca2+ entry) in RBL-2H3 cells (IC50 = 590 nM by Fura-2; peak current density pA/pF = -1.13 and -7.50, respectively, with or without 3 µM BTP2/Pyr2 by whole cell clamp) than the transient receptor potential cation channel TRPC3-mediated ROCE (receptor-operated Ca2+ entry) inTRPC3-expressing HEK293 cells (IC50 = 4.21 µM by Fura-2; pA/pF = -1.54 and -18.50, respectively, with or without 3 µM BTP2/Pyr2). A great complement to Pyr 3 (Cat. No. 648490), Pyr6, and Pyr10 in Ca2+ signaling studies.
Catalogue Number203890
Brand Family Calbiochem®
Synonyms[N-{4-[3,5-bis(Trifluoromethyl)-1H-pyrazol-1-yl]phenyl}-4-methyl-1,2,3-thiadiazole-5-carboxamide], Pyr2, TPRC3 Channel Inhibitor II
References
ReferencesSchleifier, H., et al. 2012. Br J Pharmacol In Press
Yonetoku, Y., et al. 2006. Bioorg. Med. Chem. 14, 5370.
Yonetoku, Y., et al. 2006. Bioorg. Med. Chem. 14, 4750.
Zitt, C., et al. 2004. J. Biol. Chem. 279, 12927.
Trevillyan, J.M., et al. 2001. J. Biol. Chem. 276, 48118.
Djuric, S.W., et al. 2000. J. Med. Chem. 43, 2975.
Product Information
CAS number223499-30-7
ATP CompetitiveN
FormSolid
Hill FormulaC₁₅H₉F₆N₅OS
Chemical formulaC₁₅H₉F₆N₅OS
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationCRAC Channel Inhibitor, BTP2, CAS 223499-30-7, is a cell-permeable, potent blocker of Stim1 and Orai1-coupled Ca2+ release-activated Ca2+ channel-mediated SOCE in RBL-2H3 cells (IC50 = 590 nM).
Biological Information
Primary TargetCRAC Channel
Primary Target IC<sub>50</sub>590 nM by Fura-2
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
203890-5MG 04055977204070

Documentation

CRAC Channel Inhibitor, BTP2 - CAS 223499-30-7 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

CRAC Channel Inhibitor, BTP2 - CAS 223499-30-7 - Calbiochem Certificates of Analysis

TitleLot Number
203890

References

Reference overview
Schleifier, H., et al. 2012. Br J Pharmacol In Press
Yonetoku, Y., et al. 2006. Bioorg. Med. Chem. 14, 5370.
Yonetoku, Y., et al. 2006. Bioorg. Med. Chem. 14, 4750.
Zitt, C., et al. 2004. J. Biol. Chem. 279, 12927.
Trevillyan, J.M., et al. 2001. J. Biol. Chem. 276, 48118.
Djuric, S.W., et al. 2000. J. Med. Chem. 43, 2975.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-June-2013 JSW
Synonyms[N-{4-[3,5-bis(Trifluoromethyl)-1H-pyrazol-1-yl]phenyl}-4-methyl-1,2,3-thiadiazole-5-carboxamide], Pyr2, TPRC3 Channel Inhibitor II
DescriptionA cell-permeable pyrazole that acts as a more potent blocker of Stim1 and Orai1-coupled CRAC (Ca2+ release-activated Ca2+) channel-mediated SOCE (store-operated Ca2+ entry) in RBL-2H3 cells (IC50 = 590 nM by Fura-2; peak current density pA/pF = -1.13 and -7.50, respectively, with or without 3 µM BTP2/Pyr2 by whole cell clamp) than the transient receptor potential cation channel TRPC3-mediated ROCE (receptor-operated Ca2+ entry) inTRPC3-expressing HEK293 cells (IC50 = 4.21 µM by Fura-2; pA/pF = -1.54 and -18.50, respectively, with or without 3 µM BTP2/Pyr2). A great complement to Pyr 3 (Cat. No. 648490), Pyr6, and Pyr10 in Ca2+ signaling studies.
FormSolid
Intert gas (Yes/No) Packaged under inert gas
CAS number223499-30-7
Chemical formulaC₁₅H₉F₆N₅OS
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesSchleifier, H., et al. 2012. Br J Pharmacol In Press
Yonetoku, Y., et al. 2006. Bioorg. Med. Chem. 14, 5370.
Yonetoku, Y., et al. 2006. Bioorg. Med. Chem. 14, 4750.
Zitt, C., et al. 2004. J. Biol. Chem. 279, 12927.
Trevillyan, J.M., et al. 2001. J. Biol. Chem. 276, 48118.
Djuric, S.W., et al. 2000. J. Med. Chem. 43, 2975.