Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

235425 Caspase-3 Substrate II, Fluorogenic - CAS 169332-61-0 - Calbiochem

Fluorogenic caspase-3 substrate.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Ac-DEVD-AMC

View Products on Sigmaaldrich.com
235425
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
235425-1MG Plastic ampoule 1 mg
235425-5MG Plastic ampoule 5 mg
Description
OverviewFluorogenic caspase-3 substrate. Cleavage of this substrate by caspase-3 shows Michaelis-Menten kinetics (Km = 9.7 ± 1.0 µM). Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.
Catalogue Number235425
Brand Family Calbiochem®
SynonymsAc-DEVD-AMC
References
ReferencesHermission, M., et al. 2000. Oncogene 19, 2338.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Product Information
CAS number169332-61-0
ATP CompetitiveN
FormOff-white solid
Hill FormulaC₃₀H₃₇N₅O₁₃
Chemical formulaC₃₀H₃₇N₅O₁₃
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetFluorogenic caspase-3 substrate
Primary Target IC<sub>50</sub>Km = 9.7 ± 1.0 µM for cleavage of this substrate by caspase-3
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceAc-Asp-Glu-Val-Asp-AMC
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
235425-1MG 04055977217032
235425-5MG 04055977217049

Documentation

Caspase-3 Substrate II, Fluorogenic - CAS 169332-61-0 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Caspase-3 Substrate II, Fluorogenic - CAS 169332-61-0 - Calbiochem Certificates of Analysis

TitleLot Number
235425

References

Reference overview
Hermission, M., et al. 2000. Oncogene 19, 2338.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-March-2011 RFH
SynonymsAc-DEVD-AMC
DescriptionFluorogenic CPP32/Apopain substrate. Cleavage of this substrate by CPP32/Apopain shows Michaelis-Menten kinetics (Km = 9.7 ± 1.0 µM). Cleavage can be monitored by excitation at 380 nm and emission at 460 nm.
FormOff-white solid
CAS number169332-61-0
Chemical formulaC₃₀H₃₇N₅O₁₃
Peptide SequenceAc-Asp-Glu-Val-Asp-AMC
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (10 mM)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesHermission, M., et al. 2000. Oncogene 19, 2338.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature 376, 37.