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219401 Cathepsin D, Human Liver

Cathepsin D, Human Liver, CAS 9025-26-7, is a purified native cathepsin D from human liver. Overexpression of cathepsin D in is associated with higher risk of cancer relapse and metastasis.

Cathepsin D, Human Liver MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
219401
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
219401-15U Plastic ampoule 15 u
219401-30U Plastic ampoule 30 u
Description
OverviewNative cathepsin D from human liver. A major lysosomal aspartyl protease produced as a 52 kDa proenzyme. Overexpression of cathepsin D in human breast cancers is associated with higher risk of relapse and metastasis. Cathepsin D degrades extracellular matrix and releases growth factors bound to the matrix.
Catalogue Number219401
Brand Family Calbiochem®
References
ReferencesBerchem, G., et al. 2002. Oncogene 21, 5951.
Barbi, G.P., et al. 1994. Oncology 51, 329.
Krishnan, V., et al. 1994. J. Biol. Chem. 269, 15912.
Touitou, I., et al. 1994. Eur. J. Cancer 30A, 390.
Rijnboutt, S., et al. 1991. J. Biol. Chem. 266, 23586.
Johnson, G.V., et al. 1991. J. Neurochem. 57, 1577.
Erickson, A. 1989. J. Cell. Biochem. 40, 31.
Product Information
CAS number9025-26-7
Unit of DefinitionOne unit is defined as the amount of enzyme that will digest acid-denatured hemoglobin in 60 min, at 37°C, pH 3.3, releasing peptides soluble in 10% TCA, as measured by an increase in absorbance of 1.0 at 280 nm
EC number3.4.23.5
FormLyophilized
FormulationLyophilized from 2 mM sodium phosphate buffer, pH 6.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
SourcePrepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity≥300 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C. Keep on ice when working with material. When product is brought to its active pH (3.3), autolysis occurs.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
219401-15U 04055977201710
219401-30U 04055977201727

Documentation

Cathepsin D, Human Liver Certificates of Analysis

TitleLot Number
219401

References

Reference overview
Berchem, G., et al. 2002. Oncogene 21, 5951.
Barbi, G.P., et al. 1994. Oncology 51, 329.
Krishnan, V., et al. 1994. J. Biol. Chem. 269, 15912.
Touitou, I., et al. 1994. Eur. J. Cancer 30A, 390.
Rijnboutt, S., et al. 1991. J. Biol. Chem. 266, 23586.
Johnson, G.V., et al. 1991. J. Neurochem. 57, 1577.
Erickson, A. 1989. J. Cell. Biochem. 40, 31.

Brochure

Title
Cathepsins and Related Products Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-April-2008 RFH
DescriptionNative cathepsin D from human liver. Degrades the extracellular matrix and releases growth factors bound to the matrix. Overexpression of cathepsin D in human breast cancers is associated with a higher risk of relapse and metastasis.
FormLyophilized
FormulationLyophilized from 2 mM sodium phosphate buffer, pH 6.5.
SourcePrepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number9025-26-7
EC number3.4.23.5
Purity≥90% by SDS-PAGE
Specific activity≥300 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will digest acid-denatured hemoglobin in 60 min, at 37°C, pH 3.3, releasing peptides soluble in 10% TCA, as measured by an increase in absorbance of 1.0 at 280 nm
SolubilitySterile H₂O or aqueous buffers. Please see vial label for lot-specific reconstitution volume.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C. Keep on ice when working with material. When product is brought to its active pH (3.3), autolysis occurs.
Toxicity Standard Handling
Merck USA index14, 1905
ReferencesBerchem, G., et al. 2002. Oncogene 21, 5951.
Barbi, G.P., et al. 1994. Oncology 51, 329.
Krishnan, V., et al. 1994. J. Biol. Chem. 269, 15912.
Touitou, I., et al. 1994. Eur. J. Cancer 30A, 390.
Rijnboutt, S., et al. 1991. J. Biol. Chem. 266, 23586.
Johnson, G.V., et al. 1991. J. Neurochem. 57, 1577.
Erickson, A. 1989. J. Cell. Biochem. 40, 31.