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219343 Cathepsin S, Human, Recombinant, E. coli

Cathepsin S, Human, Recombinant, E. coli, is prepared without a tag or fusion protein. A major lysosomal cysteine proteinase with high specific activity.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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219343
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Overview

Replacement Information

Products

Catalog NumberPackaging Qty/Pack
219343-25UG Plastic ampoule 25 μg
Description
OverviewRecombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase preferentially expressed in macrophages and microglia. Exhibits high specific activity.
Note: 1 mU = 1 milliunit.
Catalogue Number219343
Brand Family Calbiochem®
References
ReferencesLiuzzo, J.P., et al. 1999. Mol. Med. 5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334.
Kirschke, H., et al. 1989. Biochem. J. 264, 467.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit.
EC number3.4.22.27
FormLiquid
FormulationIn 35 mM potassium phosphate, 35 mM Sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥30,000 mU/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Harmful
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
219343-25UG 04055977202144

Documentation

Cathepsin S, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Cathepsin S, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
219343

References

Reference overview
Liuzzo, J.P., et al. 1999. Mol. Med. 5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334.
Kirschke, H., et al. 1989. Biochem. J. 264, 467.

Citations

Title
  • Ayman Marei, et al. (2005) Superior T cell activation by ESAT-6 as compared with the ESAT-6-CFP-10 complex. International Immunology 17, 1439-1446.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision07-September-2007 RFH
    DescriptionRecombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase that is preferentially expressed in macrophages and microglia. Shown to be involved in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenting cells by mediating invariant (Ii) chain degradation. Inhibition of cathepsin S results in impaired antigen-presentation.
    FormLiquid
    FormulationIn 35 mM potassium phosphate, 35 mM Sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.5.
    EC number3.4.22.27
    Purity≥90% by SDS-PAGE
    Specific activity≥30,000 mU/mg protein
    Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit.
    Storage Avoid freeze/thaw
    ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Harmful
    ReferencesLiuzzo, J.P., et al. 1999. Mol. Med. 5, 334.
    Riese, R.J., et al. 1998. J. Clin. Invest. 101, 2351.
    Sukhova, G.K., et al. 1998. J. Clin. Invest. 102, 576.
    Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol. 244, 500.
    Xin, X.Q., et al. 1992. Arch. Biochem. Biophys. 299, 334.
    Kirschke, H., et al. 1989. Biochem. J. 264, 467.
    Citation
  • Ayman Marei, et al. (2005) Superior T cell activation by ESAT-6 as compared with the ESAT-6-CFP-10 complex. International Immunology 17, 1439-1446.
    		•