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530152 Cdc42/Rac1 Dual Inhibitor, AZA1 - CAS 1071098-42-4 - Calbiochem

A cell-permeable compound that suppresses upregulation of GTP-bound Rac1 and Cdc42. Inhibits prostate cancer cells migration by decreasing cellular F-/G-actin ratio.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N²,N⁴-bis(2-Methyl-1H-indol-5-yl)pyrimidine-2,4-diamine, Cdc42 Inhibitor IV, Rac1 Inhibitor V

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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
1071098-42-4C₂₂H₂₀N₆

Products

Catalog NumberPackaging Qty/Pack
5.30152.0001 Glass bottle 10 mg
Description
OverviewA cell-permeable indolylpyrimidinediamine compound that selectively suppresses upregulation of GTP-bound Rac1 and Cdc42, but not RhoA, in prostate cancer cells upon EGF-stimulation (Effec. Conc. = 20 µM in serum-starved 22Rv1, DU 154, PC-3 cultures), resulting in time- and dose-dependent (2 to 10 µM and up to 72 h) growth inhibition in serum-deprived cultures with or without EGF stimulation. Dual Cdc42/Rac1 inhibition by AZA1 also effectively inhibits prostate cancer cells migration by decreasing cellular F-/G-actin ratio and disabling both filopodia and lamepodia formation. Daily intraperitoneal injection (100 µg/100 µL 30% DMSO/mouse) is shown to suppress 22Rv1 tumor expansion in mice in vivo.
Catalogue Number530152
Brand Family Calbiochem®
SynonymsN²,N⁴-bis(2-Methyl-1H-indol-5-yl)pyrimidine-2,4-diamine, Cdc42 Inhibitor IV, Rac1 Inhibitor V
References
ReferencesZins, K., et al. 2013. PLoS One 8, e74924.
Product Information
CAS number1071098-42-4
FormLight brown powder
Hill FormulaC₂₂H₂₀N₆
Chemical formulaC₂₂H₂₀N₆
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
5.30152.0001 04055977241235

Documentation

Cdc42/Rac1 Dual Inhibitor, AZA1 - CAS 1071098-42-4 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Cdc42/Rac1 Dual Inhibitor, AZA1 - CAS 1071098-42-4 - Calbiochem Certificates of Analysis

TitleLot Number
530152

References

Reference overview
Zins, K., et al. 2013. PLoS One 8, e74924.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-March-2014 JSW
SynonymsN²,N⁴-bis(2-Methyl-1H-indol-5-yl)pyrimidine-2,4-diamine, Cdc42 Inhibitor IV, Rac1 Inhibitor V
DescriptionA cell-permeable indolylpyrimidinediamine compound that selectively suppresses cellular levels of GTP-bound Rac1 and Cdc42, but not RhoA, in serum-starved prostate cancer cultures upon EGF-stimulation (% of non-stimulated control Rac1-GTP level with/without 20 µM AZA1 = 56/375 in 22Rv1, 30/235 in DU 154, and 12/118 in PC-3 cells; % of non-EGF-stimulated control Cdc42-GTP level with/without 20 µM AZA1 = 15/170 in 22Rv1, 30/105 in DU 154, and 12/100 in PC-3 cells), resulting in effective suppression of PAK1, Akt, and BAD phosphorylations, as well as time- and dose-dependent (2 to 10 µM and up to 72 hr) growth inhibition of the cells in serum-deprived cultures with or without EGF stimulation. Dual Cdc42/Rac1 inhibition by AZA1 also effectively inhibits prostate cancer cells migration by decreasing cellular F-/G-actin ratio and disabling both filopodia and lamepodia formation. Daily intraperitoneal injection (100 µg/100 µL 30% DMSO/mouse) is shown to be efficacious in suppressing 22Rv1 tumor expansion in mice in vivo.
FormLight brown powder
Intert gas (Yes/No) Packaged under inert gas
CAS number1071098-42-4
Chemical formulaC₂₂H₂₀N₆
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (100 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesZins, K., et al. 2013. PLoS One 8, e74924.