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14-475 Cdk2/Cyclin E Protein, active, 10 µg

Active complex of recombinant full length human Cdk2 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag. For use in Kinase Assays.

Cdk2/Cyclin E Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-475
10 µg  Also available in 250μg size as catalog # 14-475M - Call for pricing and availability.
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Overview

Replacement Information
Description
Catalogue Number14-475
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCdk2/Cyclin E Protein, active, 10 µg
OverviewComplex of recombinant full length human cdk2 containing a C-terminal His6-tag, and recombinant full-length human cyclin E containing an N-terminal GST-tag.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive complex of recombinant full length human Cdk2 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceBoth are expressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
Gene Symbol
  • CDK2
  • p33(CDK2)
Protein TargetCDK2/cyclinE
Purification MethodNi-NTA agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
SIZE: 298 amino acids; 33930 Da
SUBUNIT: Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1.
SIMILARITY: SwissProt: P24941 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight34kDa (cdk2), 74kDa (cyclin
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of histone H1 by cdk2/cyclin E
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1year at -70C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size as catalog # 14-475M - Call for pricing and availability.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-475 04053252743689

Documentation

Cdk2/Cyclin E Protein, active, 10 µg SDS

Title

Safety Data Sheet (SDS) 

Cdk2/Cyclin E Protein, active, 10 µg Certificates of Analysis

TitleLot Number
Cdk2/cyclinE, active - 1851584-E 1851584-E
Cdk2/cyclinE, active - 1851584-F 1851584-F
Cdk2/cyclinE, active - 1901980-A 1901980-A
Cdk2/cyclinE, active - 2166901-A 2166901-A
Cdk2/cyclinE, active - D9AN017U-I D9AN017U-I

FAQ

QuestionAnswer
Some of my enzyme preparations require Brij-35, what is its purpose?The role of Brij-35 is to function as a mild detergent, which prevents aggregation in active kinases.

Related Products & Applications

Alternative Packsize

Catalog Number Description
14-475M Cdk2/Cyclin E Protein, active, 250 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases