Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

14-516 Cdk5/p25 Protein, active, 10 µg

Active complex of N-terminal His6-tagged, recombinant, full-length human Cdk5 & N-terminal GST-tagged, recombinant, full-length human p25, for use in Kinase Assays.

Cdk5/p25 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
14-516
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-516M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-516
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCdk5/p25 Protein, active, 10 µg
OverviewComplex of N-terminal His6-tagged, recombinant, full-length human cdk5 and Nterminal GST-tagged, recombinant, full-length human p25
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive complex of N-terminal His6-tagged, recombinant, full-length human Cdk5 & N-terminal GST-tagged, recombinant, full-length human p25, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceCo-expressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • CDK5
  • PSSALRE
Protein TargetCDK5/p25
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q00535 # Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
SIZE: 292 amino acids; 33304 Da
SUBUNIT: Heterodimer of a catalytic subunit and a regulatory subunit (p35). Found in a trimolecular complex with CABLES1 and ABL1. Interacts with CABLES1 (By similarity). Interacts with AATK.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, growth cone (By similarity). Note=In axonal growth cone with extension to the peripheral lamellipodia (By similarity).
SIMILARITY: SwissProt: Q00535 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight34.4kDa (cdk5), 49.4kDa (p25)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Histone H1
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-516M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
14-516 04053252284465

Related Products & Applications

Alternative Packsize

Catalog Number Description
14-516M Cdk5/p25 Protein, active, 250 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases