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17-10121 ChIPAb+ Acetyl-Histone H4 (Lys12) - ChIP Validated Antibody and Primer Set, rabbit monoclonal

This ChIPAb+ Acetyl-Histone H4 (Lys12) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Cell Function Assay, Chromatin Immunoprecipitation (ChIP) Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones, Chromatin Biology
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17-10121
25 assays  25 assays per set. Recommended use: 2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
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Key Specifications Table

Species ReactivityKey Applications
H, VrtWB, Cell Function Assay, ChIP
Description
Catalogue Number17-10121
Trade Name
  • ChIPAb+
  • Upstate
DescriptionChIPAb+ Acetyl-Histone H4 (Lys12) - ChIP Validated Antibody and Primer Set, rabbit monoclonal
OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Acetyl-Histone H4 (Lys12) set includes the Acetyl-Histone H4 (Lys12) antibody, a negative control normal rabbit IgG, and qPCR primers which amplify a 213 bp region of human GAPDH coding region. The Acetyl-Histone H4 (Lys12) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Acetyl-Histone H4 (Lys12) associated chromatin.
Alternate Names
  • H4K12Ac
  • Histone H4 (acetyl K12)
References
Product Information
FormatPurified
Control
  • Includes negative control supernatant and primers specific for human GAPDH coding region.
PresentationAnti-Acetyl-Histone H4 (Lys12) (rabbit monoclonal), . One vial containing 50 µL of rabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide. Store at -20°C.
Negative Control Supernatant, . One vial containing 100 µL of cultured supernatant in 0.05% sodium azide. Store at -20°C.
ChIP Primers, human GAPDH coding region,. One vial containing 75 μL of 5 μM each primer specific for human GAPDH coding region.
Store at -20°C.
FOR: GGC TCC CAC CTT TCT CAT CC
REV: GGC CAT CCA CAG TCT TCT GG
Quality LevelMQ100
Applications
ApplicationThis ChIPAb+ Acetyl-Histone H4 (Lys12) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
Key Applications
  • Western Blotting
  • Cell Function Assay
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Negative Control Supernatant, or 2 µL of Anti-Acetyl-Histone H4 (Lys12) and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H4 (Lys12)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH Coding Region as a positive locus, and a gene desert region as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
Lysates from HeLa cells untreated or sodium butyrate treated (Lanes 1 and 2 respectively) were resolved probed with anti-acetyl-Histone H4 (Lys12) (1:1,000). Arrow indicates Acetyl-Histone H4 (Lys12).
Arrow indicates Acetyl-Histone H4 (Lys12) (~11 kDa) (Figure 3).
Biological Information
ImmunogenPeptide corresponding to Histone H4 containing the sequence [GLG-AcK-GGA] on which Lys12 is acetylated.
EpitopeUnknown
HostRabbit
SpecificityThis antibody detects histone H4 acetylated at Lys12.
IsotypeIgG
Species Reactivity
  • Human
  • Vertebrates
Species Reactivity NoteReacts with Human, wide range of cross-reactivity expected based on sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Gene Symbol
  • HIST1H4A
  • H4/J
  • HIST1H4F
  • HIST1H4L
  • H4FN
  • H4FA
  • H4FH
  • HIST1H4H
  • H4F2
  • H4FM
  • H4/A
  • H4FK
  • H4FG
  • HIST2H4
  • H4FB
  • H4/K
  • HIST1H4I
  • H4/N
  • HIST1H4B
  • H4FE
  • H4/M
  • H4/a
  • HIST1H4E
  • HIST4H4
  • HIST2H4A
  • H4FC
  • H4FI
  • H4/H
  • HIST1H4C
  • H4/G
  • HIST1H4K
  • H4FJ
  • H4FD
  • H4/I
  • H4/B
  • H4/D
  • H4/C
  • HIST1H4J
  • HIST1H4D
  • H4/E
Modifications
  • Acetylation
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Molecular Weight11kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceChromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa S3 cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Negative Control Supernatant, or 2 µL of Anti-Acetyl-Histone H4 (Lys12) and the Magna ChIP™ A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H4 (Lys12)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH promoter (Figure 1).
Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 assays
Material Package25 assays per set. Recommended use: 2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
17-10121 04053252682339

Documentation

ChIPAb+ Acetyl-Histone H4 (Lys12) - ChIP Validated Antibody and Primer Set, rabbit monoclonal SDS

Title

Safety Data Sheet (SDS) 

ChIPAb+ Acetyl-Histone H4 (Lys12) - ChIP Validated Antibody and Primer Set, rabbit monoclonal Certificates of Analysis

TitleLot Number
ChIPAb+ Acetyl-Histone H4 (Lys12) - 2197300 2197300
ChIPAb+ Acetyl-Histone H4 (Lys12) - 3285868 3285868
ChIPAb+ Acetyl-Histone H4 (Lys12) - NRG1915761 NRG1915761
ChIPAb+ Acetyl-Histone H4 (Lys12) -2548061 2548061
ChIPAb+ Acetyl-Histone H4 (Lys12) -2702120 2702120
ChIPAb+ Acetyl-Histone H4 (Lys12) Monoclonal Antibody/Primer Set 2958932
ChIPAb+ Acetyl-Histone H4 (Lys12) Monoclonal Antibody/Primer Set 3030900
ChIPAb+ Acetyl-Histone H4 (Lys12) Monoclonal Antibody/Primer Set 3082348

Brochure

Title
Advance your Epigenetics Research

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Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies