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17-10530 ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set

Use ChIPAb+ MED1 ChIP Validated rabbit polyclonal antibody for the detection of MED also known as Mediator of RNA polymerase II transcription subunit 1 & CRSP1/TRAP220.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Chromatin Immunoprecipitation (ChIP), Western Blotting Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors
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17-10530
25 assays  25 assays per set. Recommended use: 2.0 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
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Key Specifications Table

Species ReactivityKey Applications
H, MChIP, WB
Description
Catalogue Number17-10530
Trade Name
  • ChIPAb+
  • Upstate
DescriptionChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set
OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ MED1 (CRSP1/TRAP220) set includes a MED1 antibody, a Normal Rabbit IgG, and positive control primers which amplify 85 bp of a human prostate-specific antigen (PSA) enhancer region. The MED1 antibody and negative control IgG are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of MED1 associated chromatin.
Alternate Names
  • CRSP1
  • TRAP220
  • MED1
  • TRIP-2
  • ARC205
  • Mediator of RNA polymerase II transcription subunit 1
  • Activator-recruited cofactor 205 kDa component
  • Mediator complex subunit 1
  • p53 regulatory protein RB18A
  • Peroxisome proliferator-activated receptor-binding protein
  • Thyroid receptor-interacting protein 2
  • PBP
  • PPAR-binding protein
  • Thyroid hormone receptor-associated protein complex 220 kDa component
  • Trap220
  • Thyroid receptor-interacting protein 2
  • TR-interacting protein 2
  • TRIP-2
  • Vitamin D receptor-interacting protein complex component DRIP205
  • p53 regulatory protein RB18A
References
Product Information
FormatAffinity Purified
Control
  • Controls
    Includes normal rabbit IgG and primers specific for a human prostate-specific antigen enhancer region.
PresentationAnti-MED1 (Rabbit Polyclonal), Part No. CS207349. One vial containing 50 µg affinity purified rabbit polyclonal in 72 uL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of glycerol to 30% (0.7 mg/mL final). Store at -20°C.

Normal Rabbit IgG, Part No. PP64B. One vial containing 125 μg of purified Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C.

ChIP Primers, PSA. Part No. CS207340. One vial containing 75 μL of 5 μM of each primer specific for human prostate-specific antigen enhancer region (chr19:51354179-51354263, hg19 build). Store at -20°C.
FOR: GCC TGG ATC TGA GAG AGA TAT CAT C
REV: ACA CCT TTT TTT TTC TGG ATT GTT G
Quality LevelMQ100
Applications
ApplicationUse ChIPAb+ MED1 ChIP Validated rabbit polyclonal antibody for the detection of MED also known as Mediator of RNA polymerase II transcription subunit 1 & CRSP1/TRAP220.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Western Blotting
Application NotesChromatin Immunoprecipitation:
Sonicated chromatin prepared from hormone starved (72H), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (~1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No.CS207349) and the Magna ChIP™ HiSens (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340) as a positive locus, and ChIP Primers, β-globin (Part No. CS200591) as a negative locus.
Please refer to the Magna ChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens(Cat. # 17-10461) protocol for experimental details.

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from hormone starved (72H), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (1e5 and 1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No. CS207349) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340).
Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.

Western Blotting Analysis:
0.04 µg/mL of this antibody detected MED1 on 5, 15 and 50 µg of HeLa whole cell lysate.
Biological Information
ImmunogenRecombinant protein corresponding to human MED1 (a.a. 1525-1581).
EpitopeHuman MED1 (a.a. 1525-1581).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteDemonstrated to react with Human and Mouse. Wide species reactivity expected based upon conservation of immunogen sequence.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARC2052
  • CRSP1
  • CRSP200
  • DRIP205
  • DRIP230
  • PBP
  • PPARBP
  • PPARGBP
  • RB18A
  • TRAP220
  • TRIP
UniProt Number
Molecular Weight~168 kDa based on sequence. Higher mw up to 220 kDa observed on western blots.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceChromatin Immunoprecipitation:
Sonicated chromatin prepared from hormone starved (72h), then androgen agonist induced (1 µM CI-4AS-1 overnight) LNCaP cells (~1e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG (Part No. PP64B), or 2 µg Anti-MED1 (Part No.CS207349) and the Magna ChIP™ HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of MED1 associated DNA fragments was verified by qPCR using ChIP Primers, PSA (Part No. CS207340).
Please refer to the MagnaChIP™ HiSens (Cat. # 17-10460) or EZ-MagnaChIP™ HiSens (Cat. # 17-10461) protocol for experimental details.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 assays
Material Package25 assays per set. Recommended use: 2.0 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
17-10530 04053252947919

Documentation

ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set SDS

Title

Safety Data Sheet (SDS) 

ChIPAb+ MED1 Antibody - ChIP Validated Antibody and Primer Set Certificates of Analysis

TitleLot Number
ChIPAb+ MED1 - Q2370588 Q2370588
ChIPAb+ MED1 - 3176293 3176293
ChIPAb+ MED1 - 3282684 3282684
ChIPAb+ MED1 - 3386083 3386083
ChIPAb+ MED1 - 3386606 3386606
ChIPAb+ MED1 - 3503505 3503505
ChIPAb+ MED1 - 3590174 3590174
ChIPAb+ MED1 - 3789473 3789473
ChIPAb+ MED1 - 3865030 3865030
ChIPAb+ MED1 - 3931645 3931645
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Title
Advance your Epigenetics Research